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JCM 1131 . SALINISPIRA L21 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1131 #

ml
Equipment needed: Hungate tubes Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 60.0 g 58.252 996.79
MgCl2 x 6 H2O 6.0 g 5.825 28.653
KCl 1.5 g 1.456 19.535
Na2SO4 1.0 g 0.971 6.835
NH4Cl 1.0 g 0.971 18.151
CaCl2 x 2 H2O 0.4 g 0.388 2.642
K2HPO4 0.4 g 0.388 2.23
Trace mineral solution 10.0 ml - -
Yeast extract 1.0 g 0.971 -
Trypticase peptone
(BD-BBL) 		1.0 g 0.971 -
Resazurin 0.5 mg 4.9e-4 0.002
Distilled water 950.0 ml - -
1 Mix components thoroughly, bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream and autoclave under the same gas mixture. After cooling, add per liter the following solutions (autoclaved or *filter-sterilized):
Glucose
(10%) 		10.0 ml - -
Trace vitamins
(*) 		10.0 ml - -
NaHCO3
(*, 8%) 		30.0 ml - -
2 Aseptically and anaerobically, dispense the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
L-Cysteine HCl x H2O
(5%) 		10.0 ml - -
Na2S x 9 H2O
(5%) 		10.0 ml - -
3 Readjust pH to 7.3-7.5, if necessary.

Trace mineral solution #

1000
Solution occurs in 4 other media (first defined in Medium J1084).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace minerals 1000.00 ml - -
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 58.262 996.956
MgCl2 x 6 H2O 5.825 28.653
KCl 1.456 19.535
NH4Cl 0.971 18.151
Na2SO4 0.971 6.835
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain