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J1135. METHANOBACTERIUM MEDIUM FOR STRAIN CAN

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.500 g 0.476 8.148
2 0.500 g 0.476 8.902
3 0.100 g 0.095 0.547
4 0.100 g 0.095 0.7
5 0.100 g 0.095 0.648
6 0.100 g 0.095 0.386
7 FeCl2 solution 1.000 ml - -
8 Trace element solution 1.000 ml - -
9 2.000 g 1.905 28.008
10 1.000 g 0.952 11.61
11 0.002 g 0.002 0.007
12 0.100 g 0.095 0.542
13 0.500 mg 4.8e-4 0.002
14 1000.000 ml 950.673 52770.3
15
(30%)
10.000 ml - -
16
(8%)
20.000 ml 3.855 36.374
17 Trace vitamins 10.000 ml - -
18
(5%)
8.000 ml - -
Instructions:
  1. Mix components thoroughly, bring to a boil for several seconds, and cool down under a stream of N2-CO2 (80:20, v/v). Distribute the medium into culture vessels (e.g., 5 ml in the Hungate tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following sterile solutions from anaerobic stocks (autoclaved or filter-sterilized): #15-17
  2. Prior to inoculation, replace the gas phase with a gas mixture of H2-CO2 (80:20, v/v), and add per liter the following solution (autoclaved and stored under a N2 atmosphere): #18
  3. Pressurize the inoculated culture vessels to 200 kPa H2-CO2 (80:20, v/v).
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 962.072 53403
Na2CO3 3.855 36.374
Sodium formate 1.905 28.008
Sodium acetate 0.952 11.61
NH4Cl 0.476 8.902
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