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JCM 1135 . METHANOBACTERIUM MEDIUM FOR STRAIN CAN

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1135 #

ml
Equipment needed: Hungate tubes Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 0.5 g 0.476 8.148
NH4Cl 0.5 g 0.476 8.902
K2HPO4 0.1 g 0.095 0.547
KH2PO4 0.1 g 0.095 0.7
CaCl2 x 2 H2O 0.1 g 0.095 0.648
MgSO4 x 7 H2O 0.1 g 0.095 0.386
FeCl2 solution 1.0 ml - -
Trace element solution 1.0 ml - -
Sodium formate 2.0 g 1.905 28.008
Sodium acetate 1.0 g 0.952 11.61
FeSO4 x 7 H2O 2.0 mg 0.002 0.007
L-Cysteine HCl x H2O 0.1 g 0.095 -
Resazurin 0.5 mg 4.8e-4 0.002
Distilled water 1000.0 ml - -
1 Mix components thoroughly, bring to a boil for several seconds, and cool down under a stream of N2-CO2 (80:20, v/v). Distribute the medium into culture vessels (e.g., 5 ml in the Hungate tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following sterile solutions from anaerobic stocks (autoclaved or filter-sterilized):
Tris base
(30%) 		10.0 ml - -
Na2CO3
(8%) 		20.0 ml - -
Trace vitamins 10.0 ml - -
2 Prior to inoculation, replace the gas phase with a gas mixture of H2-CO2 (80:20, v/v), and add per liter the following solution (autoclaved and stored under a N2 atmosphere):
Na2S x 9 H2O
(5%) 		8.0 ml - -
3 Pressurize the inoculated culture vessels to 200 kPa H2-CO2 (80:20, v/v).

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sodium formate 1.905 28.008
Sodium acetate 0.952 11.61
NH4Cl 0.476 8.902
NaCl 0.476 8.148
KH2PO4 0.095 0.7
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