Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 0.5 | g | 0.476 | 8.148 | |
NH4Cl | 0.5 | g | 0.476 | 8.902 | |
K2HPO4 | 0.1 | g | 0.095 | 0.547 | |
KH2PO4 | 0.1 | g | 0.095 | 0.7 | |
CaCl2 x 2 H2O | 0.1 | g | 0.095 | 0.648 | |
MgSO4 x 7 H2O | 0.1 | g | 0.095 | 0.386 | |
FeCl2 solution | 1.0 | ml | - | - | |
Trace element solution | 1.0 | ml | - | - | |
Sodium formate | 2.0 | g | 1.905 | 28.008 | |
Sodium acetate | 1.0 | g | 0.952 | 11.61 | |
FeSO4 x 7 H2O | 2.0 | mg | 0.002 | 0.007 | |
L-Cysteine HCl x H2O | 0.1 | g | 0.095 | - | |
Resazurin | 0.5 | mg | 4.8e-4 | 0.002 | |
Distilled water | 1000.0 | ml | - | - | |
1 Mix components thoroughly, bring to a boil for several seconds, and cool down under a stream of N2-CO2 (80:20, v/v). Distribute the medium into culture vessels (e.g., 5 ml in the Hungate tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following sterile solutions from anaerobic stocks (autoclaved or filter-sterilized): | |||||
Tris base
(30%) |
10.0 | ml | - | - | |
Na2CO3
(8%) |
20.0 | ml | - | - | |
Trace vitamins | 10.0 | ml | - | - | |
2 Prior to inoculation, replace the gas phase with a gas mixture of H2-CO2 (80:20, v/v), and add per liter the following solution (autoclaved and stored under a N2 atmosphere): | |||||
Na2S x 9 H2O
(5%) |
8.0 | ml | - | - | |
3 Pressurize the inoculated culture vessels to 200 kPa H2-CO2 (80:20, v/v). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Medium type: | Defined medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave, Filter, Hungate tubes |
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