J1136
1136 .
IRD F1F22 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1136 #
ml
Equipment needed: Filter
Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.30 | g | 0.297 | 5.559 | |
| KH2PO4 | 0.20 | g | 0.198 | 1.457 | |
| KCl | 0.50 | g | 0.496 | 6.647 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.149 | 1.011 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.396 | 1.95 | |
| Yeast extract | 0.50 | g | 0.496 | - | |
| Trace element solution | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.496 | - | |
| Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 960.00 | ml | - | - | |
| 1 Mix components and adjust pH to 7.0. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized): | |||||
|
Glucose
(1 M) |
20.00 | ml | - | - | |
|
NaHCO3
(*, 5%) |
20.00 | ml | - | - | |
| 2 Prior to use, add per liter the following solution to reduce the medium (autoclaved and stored under N2): | |||||
|
Na2S x 9 H2O
(5%) |
8.00 | ml | - | - | |
Solution occurs in 38
other media (first defined in Medium J439).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(25%) |
12.5 | ml | - | - |
| FeSO4 x 7 H2O | 2.1 | g | 2.1 | 7.554 |
| H3BO3 | 30.0 | mg | 0.03 | 0.485 |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | - |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 |
| ZnSO4 x 7 H2O | 144.0 | mg | 0.144 | 0.501 |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
| Distilled water | 987.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |