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JCM 1136 . IRD F1F22 MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.30 g 0.297 5.559
KH2PO4 0.20 g 0.198 1.457
KCl 0.50 g 0.496 6.647
CaCl2 x 2 H2O 0.15 g 0.149 1.011
MgCl2 x 6 H2O 0.40 g 0.396 1.95
Yeast extract 0.50 g 0.496 -
Trace element solution 1.00 ml - -
L-Cysteine HCl x H2O 0.50 g 0.496 -
Resazurin 1.00 mg 9.9e-4 0.004
Distilled water 960.00 ml - -
1 Mix components and adjust pH to 7.0. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
Glucose
(1 M)
20.00 ml - -
NaHCO3
(*, 5%)
20.00 ml - -
2 Prior to use, add per liter the following solution to reduce the medium (autoclaved and stored under N2):
Na2S x 9 H2O
(5%)
8.00 ml - -
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KCl 0.496 6.647
Yeast extract 0.496 -
L-Cysteine HCl x H2O 0.496 -
MgCl2 x 6 H2O 0.396 1.95
NH4Cl 0.297 5.559
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