J1137
1137 .
ROSEIBACA CALIDILACUS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1137 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| 5 x Salts solution | 200.00 | ml | - | - | |
| NaHCO3 | 0.84 | g | 0.84 | 9.999 | |
| Glycerol | 0.46 | g | 0.46 | 4.995 | |
| KH2PO4 | 0.03 | g | 0.03 | 0.22 | |
| Na2CO3 | 0.02 | g | 0.02 | 0.189 | |
| Ammonium ferric citrate | 1.42 | mg | 0.001 | - | |
| 100 x BG-FPC solution | 10.00 | ml | - | - | |
|
TES
(pH 8.0, 1 M) |
10.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
| Distilled water | 770.00 | ml | - | - | |
| 1 Mix components thoroughly and filter-sterilize. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 493.0 | g | - | - | |
| Na2SO4 | 57.0 | g | - | - | |
| KCl | 7.5 | g | - | - | |
| 1 Add components to distilled water and bring volume to 1.0 L. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaNO3 | 149.58 | g | 149.58 | 1759.87 |
| MgSO4 x 7 H2O | 7.49 | g | 7.49 | 30.388 |
| CaCl2 x 2 H2O | 3.60 | g | 3.6 | 24.487 |
| Na2-EDTA | 0.10 | g | 0.1 | - |
| Trace element solution | 100.00 | ml | - | - |
| Distilled water | 900.00 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| H3BO3 | 2.86 | g | 2.86 | 46.248 |
| MnCl2 x 4 H2O | 1.81 | g | 1.81 | - |
| ZnSO4 x 7 H2O | 222.00 | mg | 0.222 | 0.772 |
| Na2MoO4 x 2 H2O | 390.00 | mg | 0.39 | 1.612 |
| CuSO4 x 5 H2O | 79.00 | mg | 0.079 | 0.316 |
| Ca(NO3)2 x 6 H2O | 49.40 | mg | 0.049 | - |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | n.d. |
| Equipment needed: | Filter |
| Edit in Medium builder | |