J1138
1138 .
NEUTRAL OLIGOTROPHIC HALOARCHAEAL MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1138 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MDS salt water | 767.00 | ml | - | - | |
| NH4Cl | 0.25 | g | 0.25 | 4.674 | |
| K2HPO4 | 0.30 | g | 0.3 | 1.722 | |
|
Yeast extract
(Oxoid) |
0.05 | g | 0.05 | - | |
| Peptone | 0.25 | g | 0.25 | - | |
| Sodium pyruvate | 1.00 | g | 1 | 9.088 | |
| Distilled water | 233.00 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 7.0. For preparation of solid medium, add 20.0 g/L agar (if necessary, use washed agar as described in the recipe of Medium No. 574). | |||||
Solution occurs in 5
other media (first defined in Medium J574).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 240 | g | 240 | 4106.78 | |
| MgCl2 x 6 H2O | 30 | g | 30 | 147.563 | |
| MgSO4 x 7 H2O | 35 | g | 35 | 142.002 | |
| KCl | 7 | g | 7 | 93.897 | |
|
CaCl2
(1 M) |
5 | ml | 0.555 | 5 | |
| 1 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.5 with 1 M Tris base. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Edit in Medium builder | |