Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
CaCl2 x 2 H2O | 0.080 | g | 0.077 | 0.523 | |
MgCl2 x 6 H2O | 0.200 | g | 0.192 | 0.946 | |
NaCl | 10.000 | g | 9.615 | 164.534 | |
KCl | 1.000 | g | 0.962 | 12.898 | |
NH4Cl | 1.000 | g | 0.962 | 17.976 | |
PIPES | 1.000 | g | 0.962 | 3.18 | |
Yeast extract | 0.500 | g | 0.481 | - | |
FeCl2 solution | 1.000 | ml | - | - | |
Trace element solution | 1.000 | ml | - | - | |
Trace vitamins | 1.000 | ml | - | - | |
Selenite-tungstate solution | 1.000 | ml | - | - | |
Resazurin | 1.000 | mg | 9.6e-4 | 0.004 | |
Phosphate buffer | 5.000 | ml | - | - | |
Distilled water | 1000.000 | ml | - | - | |
1 Mix components thoroughly, bring to a boil and cool down under a N2-CO2 (80:20, v/v) gas mixture. When the medium is cooled to around 50C, add the following components: | |||||
NaHCO3 | 2.000 | g | 1.923 | 22.892 | |
L-Cysteine HCl x H2O | 0.031 | g | 0.03 | - | |
2 Continue flushing with the gas mixture until the chemicals are dissolved completely. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions (autoclave and stored under a N2 atmosphere): | |||||
Glucose
(1.0 M) |
10.000 | ml | - | - | |
Ferric citrate
(pH 7.0, 0.25 M) |
20.000 | ml | - | - | |
Na2S x 9 H2O
(5%) |
0.500 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
KH2PO4 | 12.5 | g | 12.5 | 91.854 |
K2HPO4 | 20.0 | g | 20 | 114.826 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 0.4 | g | 0.4 | 10.001 |
Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Autoclave |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 9.615 | 164.534 |
NaHCO3 | 1.923 | 22.892 |
NH4Cl | 0.962 | 17.976 |
KCl | 0.962 | 12.898 |
PIPES | 0.962 | 3.18 |
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