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JCM 1143 . ORENIA METALLIREDUCENS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1143 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 1.500 g 1.437 7.067
NaCl 20.000 g 19.157 327.808
KCl 1.000 g 0.958 12.849
CaCl2 x 2 H2O 0.050 g 0.048 0.326
NH4Cl 0.500 g 0.479 8.954
K2SO4 0.440 g 0.421 2.419
KH2PO4 0.350 g 0.335 2.464
KBr 0.200 g 0.192 1.61
Yeast extract 0.500 g 0.479 -
FeCl2 solution 1.000 ml - -
Trace element solution 1.000 ml - -
Trace vitamins 1.000 ml - -
Selenite-tungstate solution 1.000 ml - -
Resazurin 1.000 mg 9.6e-4 0.004
Phosphate buffer 5.000 ml - -
Distilled water 1000.000 ml - -
1 Mix components thoroughly, bring to a boil and cool down under a N2-CO2 (80:20, v/v) gas mixture. When the medium is cooled to around 50C, add the following components:
NaHCO3 2.000 g 1.916 22.804
L-Cysteine HCl x H2O 0.031 g 0.03 -
2 Continue flushing with the gas mixture until the chemicals are dissolved completely. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions (autoclave and stored under a N2 atmosphere):
Glucose
(1.0 M) 		10.000 ml - -
Ferric citrate
(pH 7.0, 0.25 M) 		20.000 ml - -
CaCl2 x 2 H2O
(1.88 M) 		5.000 ml - -
Na2S x 9 H2O
(5%) 		0.500 ml - -

Phosphate buffer #

1000
Solution occurs in 1 other media (first defined in Medium J1142).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 12.5 g 12.5 91.854
K2HPO4 20.0 g 20 114.826
Distilled water 1000.0 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.157 327.808
NaHCO3 1.916 22.804
MgCl2 x 6 H2O 1.437 7.067
KCl 0.958 12.849
NH4Cl 0.479 8.954
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain