J1144
1144 .
ABYSSISOLIBACTER MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1144 #
ml
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 22.000 | g | 21.526 | 368.351 | |
| MgSO4 x 7 H2O | 3.450 | g | 3.376 | 13.696 | |
| MgCl2 x 6 H2O | 2.800 | g | 2.74 | 13.476 | |
| NH4Cl | 0.500 | g | 0.489 | 9.146 | |
| KCl | 0.340 | - | - | ||
| CaCl2 x 2 H2O | 0.140 | g | 0.137 | 0.932 | |
| KH2PO4 | 0.140 | g | 0.137 | 1.007 | |
| Trace minerals | 1.000 | ml | - | - | |
| Trace vitamins | 1.000 | ml | - | - | |
| Yeast extract | 2.000 | g | 1.957 | - | |
| Sodium acetate x 3 H2O | 1.400 | g | 1.37 | - | |
| FeSO4 x 7 H2O | 0.017 | g | 0.017 | 0.06 | |
| Resazurin | 1.000 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 955.000 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 7.0. Autoclave under a N2-CO2 (80:20, v/v) gas mixture. After cooling, add the following solutions (autoclaved or *filter sterilized): | |||||
|
Glucose
(10%) |
5.000 | ml | - | - | |
|
NaHCO3
(*, 8%) |
40.000 | ml | - | - | |
| 2 Aseptically and anaerobically distribute the medium into culture vessels (e.g., 20 ml in 60 ml serum bottles) under the same gas mixture and seal with butyl rubber stoppers. Prior to use, reduce the medium by the addition of the following solutions (per liter, autoclaved and stored under a N2 atmosphere): | |||||
|
L-Cysteine HCl x H2O
(5%) |
10.000 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.000 | ml | - | - | |
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 21.527 | 368.368 |
| MgSO4 x 7 H2O | 3.379 | 13.708 |
| MgCl2 x 6 H2O | 2.74 | 13.476 |
| Yeast extract | 1.957 | - |
| Sodium acetate x 3 H2O | 1.37 | - |
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