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JCM 1146 . METHANIMICROCOCCUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1146 #

ml
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.35 g 0.344 1.978
KH2PO4 0.23 g 0.226 1.663
NH4Cl 0.50 g 0.492 9.2
MgSO4 x 7 H2O 0.50 g 0.492 1.997
CaCl2 x 2 H2O 0.25 g 0.246 1.674
NaCl 2.25 g 2.215 37.895
FeSO4 x 7 H2O 2.00 mg 0.002 0.007
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Yeast extract
(Oxoid) 		2.00 g 1.969 -
Casitone
(BD-BBL) 		2.00 g 1.969 -
Sodium acetate 1.00 g 0.984 11.999
Resazurin 0.50 mg 4.9e-4 0.002
Distilled water 925.00 ml - -
1 Mix components and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (autoclaved or filter-sterilized):
NaHCO3
(*, 8%) 		35.00 ml - -
Methanol
(v/v, 50%) 		20.00 ml - -
Coenzyme M
(*, 1.4%) 		10.00 ml - -
Trace vitamins
(*) 		10.00 ml - -
2 Aseptically and anaerobically distribute the medium into culture vessels under a H2-CO2 (80:20, v/v) gas stream and seal with butyl stoppers. Prior to inoculation, add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
Na2S x 9 H2O
(5%) 		7.20 ml - -
L-Cysteine HCl x H2O
(5%) 		7.20 ml - -
3 Readjust pH to 7.0 - 7.2, if necessary. Pressurize inoculated culture vessels to 100 kPa H2-CO2 (80:20, v/v).

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.1
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 2.215 37.895
Casitone 1.969 -
Yeast extract 1.969 -
Sodium acetate 0.984 11.999
NH4Cl 0.492 9.2
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain