MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 1147 . METHANOBACTERUM MEDIUM (IX)

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1147 #

ml
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.49 3.599
MgSO4 x 7 H2O 0.40 g 0.392 1.59
NaCl 0.40 g 0.392 6.704
NH4Cl 0.40 g 0.392 7.324
CaCl2 x 2 H2O 0.05 g 0.049 0.333
FeSO4 x 7 H2O 2.00 mg 0.002 0.007
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Yeast extract 1.00 g 0.979 -
Sodium acetate 1.00 g 0.979 11.94
Sodium formate 2.00 g 1.959 28.804
Fatty acid mixture 20.00 ml - -
Resazurin 0.50 mg 4.9e-4 0.002
Distilled water 922.00 ml - -
1 Mix components and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (autoclaved or filter-sterilized):
Selenite-tungstate solution
(*) 		0.50 ml - -
NaHCO3
(*, 8%) 		50.00 ml - -
Methanol
(v/v, 50%) 		6.30 ml - -
2 Aseptically and anaerobically distribute the medium into culture vessels under a H2-CO2 (80:20, v/v) gas stream and seal with butyl stoppers. Prior to inoculation, add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
Na2S x 9 H2O
(5%) 		10.00 ml - -
L-Cysteine HCl x H2O
(5%) 		10.00 ml - -
3 Readjust pH to 7.5, if necessary. Pressurize inoculated culture vessels to 100 kPa H2-CO2 (80:20, v/v).

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Fatty acid mixture #

20
Solution occurs in 5 other media (first defined in Medium J266).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Valeric acid 0.5 g 25 244.786
Isovaleric acid 0.5 g 25 244.786
alpha-Methylbutyric acid 0.5 g 25 244.786
Isobutyric acid 0.5 g 25 283.736
Distilled water 20.0 ml - -
1 Adjust pH to 7.5 with concentrated NaOH solution.

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sodium formate 1.959 28.804
Sodium acetate 0.979 11.94
Yeast extract 0.979 -
Isobutyric acid 0.49 5.558
Valeric acid 0.49 4.795
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain