Culture medium recipe
Vol.
×
Main sol. J1149 #
ml
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.50 | g | 0.495 | 3.638 | |
| MgSO4 x 7 H2O | 0.40 | g | 0.396 | 1.607 | |
| NaCl | 5.00 | g | 4.951 | 84.711 | |
| NH4Cl | 1.00 | g | 0.99 | 18.51 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.337 | |
| Sodium acetate x 3 H2O | 2.70 | g | 2.673 | - | |
| Trace element solution | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.495 | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 980.00 | ml | - | - | |
| 1 Mix components and adjust pH to 7.0. Bring to a boil and cool down under a H2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
20.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
8.00 | ml | - | - | |
Solution occurs in 38
other media (first defined in Medium J439).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(25%) |
12.5 | ml | - | - |
| FeSO4 x 7 H2O | 2.1 | g | 2.1 | 7.554 |
| H3BO3 | 30.0 | mg | 0.03 | 0.485 |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | - |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 |
| ZnSO4 x 7 H2O | 144.0 | mg | 0.144 | 0.501 |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
| Distilled water | 987.0 | ml | - | - |
Solution occurs in 71
other media (first defined in Medium J431).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaOH | 0.4 | g | 0.4 | 10.001 |
| Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
| Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
No comments for this medium.