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JCM 1149 . METHANOMASSILIICOCCUS ALVUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.495 3.638
MgSO4 x 7 H2O 0.40 g 0.396 1.607
NaCl 5.00 g 4.951 84.711
NH4Cl 1.00 g 0.99 18.51
CaCl2 x 2 H2O 0.05 g 0.05 0.337
Sodium acetate x 3 H2O 2.70 g 2.673 -
Trace element solution 1.00 ml - -
L-Cysteine HCl x H2O 0.50 g 0.495 -
Selenite-tungstate solution 1.00 ml - -
Resazurin 1.00 mg 9.9e-4 0.004
Distilled water 980.00 ml - -
1 Mix components and adjust pH to 7.0. Bring to a boil and cool down under a H2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
NaHCO3
(*, 8%)
20.00 ml - -
Na2S x 9 H2O
(5%)
8.00 ml - -
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 4.951 84.711
Sodium acetate x 3 H2O 2.673 -
NH4Cl 0.99 18.51
KH2PO4 0.495 3.638
L-Cysteine HCl x H2O 0.495 -
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