Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
KH2PO4 | 0.50 | g | ||
|
MgSO4 x 7 H2O | 0.40 | g | ||
|
NaCl | 5.00 | g | ||
|
NH4Cl | 1.00 | g | ||
|
CaCl2 x 2 H2O | 0.05 | g | ||
|
Sodium acetate x 3 H2O | 2.70 | g | ||
|
Trace element solution | 1.00 | ml | ||
|
L-Cysteine HCl x H2O | 0.50 | g | ||
|
Selenite-tungstate solution | 1.00 | ml | ||
|
Resazurin | 1.00 | mg | ||
|
Distilled water | 980.00 | ml | ||
1 Mix components and adjust pH to 7.0. Bring to a boil and cool down under a H2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
20.00 | ml | ||
|
Na2S x 9 H2O
(5%) |
8.00 | ml |
Compound | Amount | Unit | |
---|---|---|---|
|
HCl
(25%) |
12.5 | ml |
|
FeSO4 x 7 H2O | 2.1 | g |
|
H3BO3 | 30.0 | mg |
|
MnCl2 x 4 H2O | 100.0 | mg |
|
CoCl2 x 6 H2O | 190.0 | mg |
|
NiCl2 x 6 H2O | 24.0 | mg |
|
CuCl2 x 2 H2O | 2.0 | mg |
|
ZnSO4 x 7 H2O | 144.0 | mg |
|
Na2MoO4 x 2 H2O | 36.0 | mg |
|
Distilled water | 987.0 | ml |
Compound | Amount | Unit | |
---|---|---|---|
|
NaOH | 0.4 | g |
|
Na2SeO3 x 5 H2O | 6.0 | mg |
|
Na2WO4 x 2 H2O | 8.0 | mg |
|
Distilled water | 1000.0 | ml |