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JCM 1150 . MEDIUM FOR MARINE SPIROCHAETES

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1150 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.5 g 0.481 8.988
KH2PO4 0.1 g 0.096 0.707
NaCl 40.0 g 38.462 658.137
MgCl2 x 6 H2O 4.0 g 3.846 18.918
CaCl2 x 2 H2O 1.0 g 0.962 6.54
Yeast extract 1.0 g 0.962 -
Glucose 1.7 g 1.635 9.073
Trace mineral solution 1.0 ml - -
Se/W solution 1.0 ml - -
Trace vitamins solution 1.0 ml - -
Resazurin 1.0 mg 9.6e-4 0.004
Distilled water 1000.0 ml - -
1 Mix components and adjust pH to 7.5. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following solutions (autoclaved or *filter-sterilized):
NaHCO3
(*, 8%) 		25.0 ml - -
L-Cysteine HCl x H2O
(5%) 		6.0 ml - -
Na2S x 9 H2O
(5%) 		6.0 ml - -

Trace mineral solution #

1000
Solution occurs in 8 other media (first defined in Medium J852).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl2 x 4 H2O 1.990 g 1.99 10.01
MnCl2 x 4 H2O 0.198 g 0.198 -
CoCl2 x 6 H2O 0.130 g 0.13 1.001
ZnCl2 0.136 g 0.136 0.998
CuCl2 x 2 H2O 1.700 mg 0.002 0.01
H3BO3 6.200 mg 0.006 0.1
Na2MoO4 x 2 H2O 0.024 g 0.024 0.099
NiCl2 x 6 H2O 0.013 g 0.013 0.1
AlCl3 0.013 g 0.013 0.097
Distilled water 1000.000 ml - -

Se/W solution #

1000
Solution occurs in 3 other media (first defined in Medium J852).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 x 5 H2O 1.7 mg 0.002 0.006
Na2WO4 x 2 H2O 3.3 mg 0.003 0.01
Distilled water 1000.0 ml - -

Trace vitamins solution #

1000
Solution occurs in 9 other media (first defined in Medium J284).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.9 mg 0.005 0.02
Folic acid 8.8 mg 0.009 0.02
Pyridoxine hydrochloride 4.1 mg 0.004 -
Thiamine HCl 6.7 mg 0.007 -
Riboflavin 7.5 mg 0.008 0.02
Nicotinic acid 2.5 mg 0.003 0.02
DL-Calcium pantothenate 9.5 mg 0.01 0.02
Vitamin B12 27.1 mg 0.027 0.02
p-Aminobenzoic acid 2.7 mg 0.003 0.02
Lipoic acid 4.1 mg 0.004 0.02
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 38.462 658.137
MgCl2 x 6 H2O 3.846 18.918
Glucose 1.635 9.073
CaCl2 x 2 H2O 0.962 6.54
Yeast extract 0.962 -
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
nutrition chemoorganotroph 1 strain
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