Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 1.00 | g | 0.973 | 16.646 | |
KCl | 0.50 | g | 0.486 | 6.524 | |
MgCl2 x 6 H2O | 0.40 | g | 0.389 | 1.914 | |
KH2PO4 | 0.20 | g | 0.195 | 1.43 | |
NH4Cl | 0.25 | g | 0.243 | 4.546 | |
CaCl2 x 2 H2O | 0.15 | g | 0.146 | 0.993 | |
Na2SO4 | 1.42 | g | 1.381 | 9.725 | |
FeCl2 solution | 1.00 | ml | - | - | |
Trace element solution | 1.00 | ml | - | - | |
Selenite-tungstate solution | 1.00 | ml | - | - | |
Resazurin | 0.40 | mg | 3.9e-4 | 0.002 | |
Distilled water | 970.00 | ml | - | - | |
1 Mix components and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (filter-sterilized): | |||||
Vitamin solution | 1.00 | ml | - | - | |
NaHCO3
(8%) |
30.00 | ml | - | - | |
2 Aseptically and anaerobically distribute the medium into culture vessels (e.g., 50 ml of the medium in 120 ml serum bottle) under a N2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Prior to use, add per liter the following solution (autoclaved and stored under a N2 atmosphere): | |||||
Naphthalene solution
(1.5%) |
20.00 | ml | - | - | |
Na2S x 9 H2O
(5%) |
4.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Naphthalene | 1.5 | g | 15 | 117.032 |
2,2,4,4,6,8,8-Heptamethylnonane | 100.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 0.4 | g | 0.4 | 10.001 |
Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Biotin | 20 | mg | 0.02 | 0.082 | |
Nicotinic acid | 200 | mg | 0.2 | 1.625 | |
p-Aminobenzoic acid | 100 | mg | 0.1 | 0.729 | |
Thiamine HCl | 200 | mg | 0.2 | - | |
DL-Calcium pantothenate | 100 | mg | 0.1 | 0.21 | |
Pyridoxine hydrochloride | 500 | mg | 0.5 | - | |
Vitamin B12 | 100 | mg | 0.1 | 0.074 | |
Riboflavin | 100 | mg | 0.1 | 0.266 | |
Distilled water | 1000 | ml | - | - | |
1 Filter-sterilize and store under a N2 atmosphere. |
Last modified: | 29.10.21 |
Source: | JCM |
Medium type: | Defined medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
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