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JCM 1157 . DESULFONATRONUM PAR180 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1157 #

ml
Equipment needed: Hungate tubes Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 5.00 g 2.461 42.105
KH2PO4 0.20 g 0.098 0.723
Na2SO4 4.00 g 1.969 13.859
Na2CO3 3.50 g 1.722 16.251
NH4Cl 0.25 g 0.123 2.3
MgCl2 x 6 H2O 0.10 g 0.049 0.242
KCl 0.20 g 0.098 1.32
Yeast extract 0.20 g 0.098 -
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Fe-Ni-WO-Se solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Mix components thoroughly and adjust pH to 9.0. Bring to a boil and cool under a N2 gas stream. Distribute the medium into culture vessels (e.g., 10 ml of the medium in the Hungate tubes) under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized and stored under a N2 atmosphere):
Vitamin solution
(see below, *) 		1.00 ml - -
Sodium lactate
(1 M) 		20.00 ml - -
Na2S x 9 H2O
(5%) 		8.00 ml - -
2 Vitamin solution:
Thiamine HCl 10.00 mg 0.005 -
Nicotinic acid 20.00 mg 0.01 0.08
DL-Calcium pantothenate 5.00 mg 0.002 0.005
Pyridoxine hydrochloride 50.00 mg 0.025 -
Biotin 2.00 mg 9.8e-4 0.004
p-Aminobenzoic acid 10.00 mg 0.005 0.036
Vitamin B12 20.00 mg 0.01 0.007
Distilled water 1000.00 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Fe-Ni-WO-Se solution #

1000
Solution occurs in 3 other media (first defined in Medium J1121).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 1.42 g 1.42 5.108
NiSO4 x 7 H2O 1.60 g 1.6 10.339
Na2WO4 x 2 H2O 38.00 mg 0.038 0.115
Na2SeO3 x 5 H2O 3.00 mg 0.003 0.011
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.0
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 2.461 42.105
Na2SO4 1.969 13.859
Na2CO3 1.722 16.251
NH4Cl 0.123 2.3
KCl 0.098 1.32
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Cultivation metadata from 1 strains BacDive

halophily halotolerant 1 strain
pH 8 - 10 1 strain
pH 10 - 12 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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