1157 .
DESULFONATRONUM PAR180 MEDIUM
What is this page?
Selected strain: Desulfonatronum parangueonense DSM 103602
This strain uses the standard medium without modifications.
Equipment needed:
Anaerobic workbench
Autoclave
Filter
Hungate tubes
Final pH:
9.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 5.00 | g | ||
|
|
KH2PO4 | 0.20 | g | ||
|
|
Na2SO4 | 4.00 | g | ||
|
|
Na2CO3 | 3.50 | g | ||
|
|
NH4Cl | 0.25 | g | ||
|
|
MgCl2 x 6 H2O | 0.10 | g | ||
|
|
KCl | 0.20 | g | ||
|
|
Yeast extract | 0.20 | g | ||
|
|
FeCl2 solution | 1.00 | ml | ||
|
|
Trace element solution | 1.00 | ml | ||
|
|
Fe-Ni-WO-Se solution | 1.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Mix components thoroughly and adjust pH to 9.0. Bring to a boil and cool under a N2 gas stream. Distribute the medium into culture vessels (e.g., 10 ml of the medium in the Hungate tubes) under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized and stored under a N2 atmosphere): | |||||
|
|
Vitamin solution
(see below, *) |
1.00 | ml | ||
|
|
Sodium lactate
(1 M) |
20.00 | ml | ||
|
|
Na2S x 9 H2O
(5%) |
8.00 | ml | ||
| 2 Vitamin solution: | |||||
|
|
Thiamine HCl | 10.00 | mg | ||
|
|
Nicotinic acid | 20.00 | mg | ||
|
|
DL-Calcium pantothenate | 5.00 | mg | ||
|
|
Pyridoxine hydrochloride | 50.00 | mg | ||
|
|
Biotin | 2.00 | mg | ||
|
|
p-Aminobenzoic acid | 10.00 | mg | ||
|
|
Vitamin B12 | 20.00 | mg | ||
|
|
Distilled water | 1000.00 | ml | ||
FeCl2 solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(7.7 M, 25%) |
10.0 | ml |
|
|
FeCl2 x 4 H2O | 1.5 | g |
|
|
Distilled water | 990.0 | ml |
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnCl2 | 70 | mg |
|
|
MnCl2 x 4 H2O | 100 | mg |
|
|
H3BO3 | 6 | mg |
|
|
CoCl2 x 6 H2O | 190 | mg |
|
|
CuCl2 x 2 H2O | 2 | mg |
|
|
NiCl2 x 6 H2O | 24 | mg |
|
|
Na2MoO4 x 2 H2O | 36 | mg |
|
|
Distilled water | 1000 | ml |
Fe-Ni-WO-Se solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FeSO4 x 7 H2O | 1.42 | g |
|
|
NiSO4 x 7 H2O | 1.60 | g |
|
|
Na2WO4 x 2 H2O | 38.00 | mg |
|
|
Na2SeO3 x 5 H2O | 3.00 | mg |
|
|
Distilled water | 1000.00 | ml |