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JCM 1158 . GHIORSEA BIVORA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1158 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Artificial saltwater 1000.00 ml - -
Wolfe's mineral solution 1.00 ml - -
NaHCO3 1.68 g 0.839 9.989
1 Mix components thoroughly. Gas with N2-CO2 (4:1, v/v) to establish pH 6.5. Dispense the medium in culture vessels (e.g., 20 ml in 30 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling add per liter the following solution (filter-sterilized):
Modified trace vitamins
(see below) 		1.00 ml - -
2 Modified trace vitamins:
Biotin 2.00 mg 1.0e-3 0.004
Folic acid 2.00 mg 1.0e-3 0.002
Pyridoxine hydrochloride 10.00 mg 0.005 -
Thiamine HCl 5.00 mg 0.002 -
Riboflavin 5.00 mg 0.002 0.007
Nicotinic acid 5.00 mg 0.002 0.02
DL-Calcium pantothenate 5.00 mg 0.002 0.005
Vitamin B12 0.10 mg 5.0e-5 3.7e-5
p-Aminobenzoic acid 5.00 mg 0.002 0.018
Lipoic acid 5.00 mg 0.002 0.012
KH2PO4 0.90 g 0.45 3.303
Distilled water 1000.00 ml - -
3 Prior to inoculation, add filter-sterilized 5% H2 and 5% air to the gas phase (by volume). Incubate statically. Replenish H2 and air every two days.

Artificial saltwater #

1000
Solution occurs in 3 other media (first defined in Medium J629).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 27.50 g 27.5 470.568
MgCl2 x 6 H2O 5.38 g 5.38 26.463
MgSO4 x 7 H2O 6.78 g 6.78 27.508
KCl 0.72 g 0.72 9.658
NaHCO3 0.20 g 0.2 2.381
CaCl2 x 2 H2O 1.40 g 1.4 9.523
NH4Cl 1.00 g 1 18.695
KH2PO4 0.05 g 0.05 0.367
Distilled water 1000.00 ml - -

Wolfe's mineral solution #

1000
Solution occurs in 7 other media (first defined in Medium J265).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace minerals 1000.00 ml - -
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2SeO3 1.00 mg 1.0e-3 -
Na2WO4 x 2 H2O 0.01 g 0.01 0.03

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Gas composition

  • 5 % H2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 13.737 235.057
MgSO4 x 7 H2O 3.388 13.746
MgCl2 x 6 H2O 2.687 13.218
NaHCO3 0.939 11.178
CaCl2 x 2 H2O 0.699 4.757
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain