J1161
1161 .
CYCLONATRUM PROTEILYTICUM MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1161 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2CO3 | 35 | g | 1750 | 16511.3 | |
| NaHCO3 | 25 | g | 1250 | 14879.7 | |
| K2HPO4 | 1 | g | 50 | 287.066 | |
| 1 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 9.5 with HCl and autoclave. After cooling, aseptically add per liter the following solutions (autoclaved or *filter-sterilized): | |||||
|
MgCl2
(1 M) |
1 | ml | - | - | |
| Trace element solution | 1 | ml | - | - | |
|
Trace vitamins
(*) |
1 | ml | - | - | |
|
Tryptone
(10%) |
15 | ml | - | - | |
|
Yeast extract
(1%) |
2 | ml | - | - | |
Solution occurs in 13
other media (first defined in Medium J1079).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 5.00 | g | 5 | 17.109 | |
| FeSO4 x 7 H2O | 2.00 | g | 2 | 7.194 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | - | |
| H3BO3 | 0.30 | g | 0.3 | 4.851 | |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
| CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 | |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 3.6. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 9.5 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |