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JCM 1162 . NAS-02 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1162 #

ml
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.2 g 0.19 1.44
KH2PO4 3.0 g 2.854 20.975
Modified Allen's trace metal solution 1.0 ml - -
Na2S2O3 x 5 H2O 2.5 g 2.379 9.584
Distilled water 1000.0 ml - -
1 Mix components thoroughly and adjust pH to 5.2. Autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions anaerobically and aseptically (autoclaved or *filter-sterilized and store under a N2 atmosphere):
MgSO4 x 7 H2O
(w/v, 5%) 		10.0 ml - -
CaCl2 x 2 H2O
(w/v, 5%) 		5.0 ml - -
Trace vitamins
(*) 		10.0 ml - -
Yeast extract
(w/v, 10%) 		5.0 ml - -
L-Cysteine HCl
(25 mM) 		10.0 ml - -
FeCl2
(130 mM) 		10.0 ml - -

Modified Allen's trace metal solution #

1000
Solution occurs in 1 other media (first defined in Medium J1014).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnCl2 x 4 H2O 1.80 g 1.8 -
Na2B4O7 x 10 H2O 4.50 g 4.5 -
ZnSO4 x 7 H2O 0.22 g 0.22 0.765
CuCl2 x 2 H2O 0.05 g 0.05 0.293
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
VOSO4 x n H2O 0.03 g 0.03 -
CoSO4 x 7 H2O 0.01 g 0.01 0.065
Distilled water 1000.00 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 5.2
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KH2PO4 2.854 20.975
Na2S2O3 x 5 H2O 2.379 9.584
(NH4)2SO4 0.19 1.44
Na2B4O7 x 10 H2O 0.004 -
MnCl2 x 4 H2O 0.002 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain