J1163
1163 .
MODIFIED BAAR'S MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1163 #
ml
Equipment needed: Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trisodium citrate x 2 H2O | 5.0 | g | 5 | - | |
| MgSO4 x 7 H2O | 2.0 | g | 2 | 8.114 | |
| CaSO4 | 1.0 | g | 1 | 7.345 | |
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| Yeast extract | 1.0 | g | 1 | - | |
| (NH4)2Ni(SO4)2 x 6 H2O | 1.0 | g | 1 | 2.532 | |
| Sodium lactate | 3.5 | g | 3.5 | 31.233 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 7.2. Bring to a boil, cool down and dispense the medium into culture vessels (e.g., 10 ml medium in Hungate tubes) under a N2 gas stream, seal with butyl rubber stoppers and autoclave. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave, Hungate tubes |
| Edit in Medium builder | |