J1171
1171 .
M39 METHYLOMONAS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1171 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 0.099 | g | 0.033 | 0.134 | |
| CaCl2 x 2 H2O | 0.147 | g | 0.049 | 0.333 | |
| K2SO4 | 0.349 | g | 0.116 | 0.666 | |
| NaNO3 | 0.170 | g | 0.057 | 0.665 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Mix components thoroughly. For preparation of solid medium, add 15.0 g/L agarose. Autoclave and, after cooling to 60C, add the following solutions (autoclaved or *filter-sterilized): | |||||
|
KH2PO4
(1.0 M) |
1.000 | ml | - | - | |
|
Selenite-tungstate solution
(*) |
1.000 | ml | - | - | |
|
Trace element solution
(see below, *) |
1.000 | ml | - | - | |
|
Vitamins solution
(see below, *) |
0.100 | ml | - | - | |
|
CeCl3
(*, 0.2 M) |
0.100 | ml | - | - | |
|
NaHCO3
(*, 1.0 M) |
3.000 | ml | - | - | |
| 2 Trace element solution: | |||||
| Na2-EDTA | 3.000 | g | 0.998 | - | |
| FeCl2 x 4 H2O | 1.500 | g | 0.499 | 2.51 | |
| SL-6 trace element solution | 1000.000 | ml | - | - | |
| 3 Vitamins solution: | |||||
| Biotin | 0.100 | mg | 3.3e-5 | 1.4e-4 | |
| Folic acid | 0.100 | mg | 3.3e-5 | 7.5e-5 | |
| Pyridoxine hydrochloride | 0.500 | mg | 1.7e-4 | - | |
| Thiamine HCl | 0.250 | mg | 8.3e-5 | - | |
| Riboflavin | 0.250 | mg | 8.3e-5 | 2.2e-4 | |
| Nicotinic acid | 0.250 | mg | 8.3e-5 | 6.8e-4 | |
| Calcium pantothenate | 0.250 | mg | 8.3e-5 | 1.7e-4 | |
| Vitamin B12 | 0.050 | mg | 1.7e-5 | 1.2e-5 | |
| p-Aminobenzoic acid | 0.250 | mg | 8.3e-5 | 6.1e-4 | |
| Lipoic acid | 0.250 | mg | 8.3e-5 | 4.0e-4 | |
| 1,4-Naphthoquinone | 0.200 | mg | 6.7e-5 | 4.2e-4 | |
| Nicotinamide | 0.250 | mg | 8.3e-5 | 6.8e-4 | |
| Hemin | 0.050 | mg | 1.7e-5 | 2.6e-5 | |
| Distilled water | 1000.000 | ml | - | - | |
| 4 Adjust pH to 6.5 - 7.0 with 0.2 N H2SO4 solution (autoclaved). Cultivate under an air containing 10% (v/v) methane in gas tight flasks or jars leaving &; 80% gas phase by volume. | |||||
Solution occurs in 71
other media (first defined in Medium J431).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaOH | 0.4 | g | 0.4 | 10.001 |
| Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
| Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 10
other media (first defined in Medium J167).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
| H3BO3 | 0.30 | g | 0.3 | 4.851 | |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
| CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 | |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 3.6. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |