J1173
1173 .
METHYLOTENERA MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1173 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 25.00 | g | 24.486 | 418.99 | |
| K2HPO4 | 0.14 | g | 0.137 | 0.787 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.137 | 0.933 | |
| MgSO4 x 7 H2O | 3.40 | g | 3.33 | 13.511 | |
| MgCl2 x 6 H2O | 4.18 | g | 4.094 | 20.138 | |
| KCl | 0.33 | g | 0.323 | 4.336 | |
| Fe(NH4)2(SO4)2 x 6 H2O | 10.00 | mg | 0.01 | - | |
| Na2WO4 x 2 H2O | 1.10 | mg | 0.001 | 0.003 | |
| NiCl2 x 6 H2O | 0.50 | mg | 4.9e-4 | 0.004 | |
| Na2SeO3 x 5 H2O | 0.50 | mg | 4.9e-4 | 0.002 | |
| CuSO4 x 5 H2O | 0.25 | mg | 2.4e-4 | 9.8e-4 | |
| Trace minerals | 10.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 7.6 and autoclave. For preparation of solid medium, add 15.0 g/L agar. After cooling, aseptically add the following solutions (autoclaved in a tightly stoppered bottle or *filter-sterilized): | |||||
|
Methanol
(v/v, 50%) |
10.00 | ml | - | - | |
|
Trace vitamins
(*) |
1.00 | ml | - | - | |
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | 7.6 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |