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JCM 1175 . CAENICOLA MOBILE MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1175 #

ml
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.495 3.638
K2HPO4 0.50 g 0.495 2.842
NH4Cl 0.50 g 0.495 9.255
NaCl 0.40 g 0.396 6.777
MgCl2 x 6 H2O 0.30 g 0.297 1.461
CaCl2 x 2 H2O 0.05 g 0.05 0.337
KCl 0.40 g 0.396 5.312
Yeast extract 2.00 g 1.98 -
Tryptone
(BD-Difco) 		2.00 g 1.98 27.859
Casamino acids
(BD-Difco) 		2.00 g 1.98 -
L-Cysteine 2.40 g 2.376 19.612
L-Arginine 4.20 g 4.158 23.872
L-Histidine 4.20 g 4.158 26.803
Trace metal solution 1.00 ml - -
Resazurin 1.00 mg 9.9e-4 0.004
Distilled water 975.00 ml - -
1 Mix components thoroughly, adjust pH to 7.5 with KOH and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (filter-sterilized):
NaHCO3
(8%) 		25.00 ml - -
Trace vitamins 1.00 ml - -
2 Aseptically and anaerobically distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Then, add per liter the following solution (autoclaved and stored under N2):
Na2S x 9 H2O
(5%) 		8.00 ml - -
3 Readjust pH to 7.5, if necessary.

Trace metal solution #

1010
Solution occurs in 3 other media (first defined in Medium J294).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(32%) 		10.000 ml 3.168 86.898
FeCl2 x 4 H2O 2.000 g 1.98 9.96
CoCl2 x 6 H2O 0.250 g 0.248 1.906
MnCl2 x 4 H2O 0.100 g 0.099 0.5
ZnCl2 0.070 g 0.069 0.508
H3BO3 6.000 mg 0.006 0.096
Na2MoO4 x 2 H2O 0.040 g 0.04 0.164
NiCl2 x 6 H2O 0.070 g 0.069 0.535
CuCl2 x 2 H2O 2.000 mg 0.002 0.012
AlCl3 0.025 g 0.025 0.186
Na2WO4 x 2 H2O 6.000 mg 0.006 0.018
Distilled water 1000.000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
L-Histidine 4.158 26.803
L-Arginine 4.158 23.872
L-Cysteine 2.376 19.612
Tryptone 1.98 27.859
Casamino acids 1.98 -
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