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J1175. CAENICOLA MOBILE MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.50 g 0.495 3.638
2 0.50 g 0.495 2.842
3 0.50 g 0.495 9.255
4 0.40 g 0.396 6.777
5 0.30 g 0.297 1.461
6 0.05 g 0.05 0.337
7 0.40 g 0.396 5.312
8 2.00 g 1.98 -
9
(BD-Difco)
2.00 g 1.98 -
10
(BD-Difco)
2.00 g 1.98 -
11 2.40 g 2.376 19.613
12 4.20 g 4.158 23.871
13 4.20 g 4.158 26.802
14 Trace metal solution 1.00 ml - -
15 1.00 mg 9.9e-4 0.004
16 975.00 ml 963.616 53488.7
17
(8%)
25.00 ml 4.275 50.892
18 Trace vitamins 1.00 ml - -
19
(5%)
8.00 ml - -
Instructions:
  1. Mix components thoroughly, adjust pH to 7.5 with KOH and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (filter-sterilized): #17-18
  2. Aseptically and anaerobically distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Then, add per liter the following solution (autoclaved and stored under N2): #19
  3. Readjust pH to 7.5, if necessary.
Solution occurs in 3 other media (first defined in medium J294 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(32%)
10.000 ml 4.017 110.185
2 2.000 g 1.98 -
3 0.250 g 0.248 -
4 0.100 g 0.099 -
5 0.070 g 0.069 0.509
6 0.006 g 0.006 0.096
7 0.040 g 0.04 0.164
8 0.070 g 0.069 -
9 0.002 g 0.002 -
10 0.025 g 0.025 0.186
11 0.006 g 0.006 -
12 1000.000 ml 988.324 54860.2
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 965.583 53597.9
NaHCO3 4.275 50.892
L-Histidine 4.158 26.802
L-Arginine 4.158 23.871
L-Cysteine 2.376 19.613
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