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J1176. METHANOSARCINA JL01 MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.15 g 0.144 1.058
2 0.29 g 0.278 1.598
3 1.00 g 0.96 17.941
4 0.20 g 0.192 -
5 0.10 g 0.096 0.653
6 0.90 g 0.864 14.779
7 FeCl2 solution 1.00 ml - -
8 Trace element solution 1.00 ml - -
9
(Oxoid)
2.00 g 1.919 -
10 0.50 mg 4.8e-4 0.002
11 2.00 g 1.919 22.848
12 1000.00 ml 957.972 53175.5
13 Trace vitamins
(*)
10.00 ml - -
14
(v/v, 50%)
10.00 ml 3.791 118.307
15
(5%)
10.00 ml - -
16
(5%)
10.00 ml - -
Instructions:
  1. Mix components except NaHCO3 and bring to a boil for a few seconds. After cooling down under a stream of N2-CO2 (80:20, v/v), add NaHCO3 to the medium. Dispense the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Allow to stand the medium until re-dissolved. Then, add per liter the following solutions (autoclaved or *filter-sterilized): #13-16
  2. Adjust pH of the medium to 6.8-7.0, if necessary.
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 969.458 53813.1
Methanol 3.791 118.307
NaHCO3 1.919 22.848
Yeast extract 1.919 -
NH4Cl 0.96 17.941
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