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JCM 1176 . METHANOSARCINA JL01 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1176 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.15 g 0.144 1.058
K2HPO4 0.29 g 0.278 1.598
NH4Cl 1.00 g 0.96 17.942
MgCl2 x 2 H2O 0.20 g 0.192 1.462
CaCl2 x 2 H2O 0.10 g 0.096 0.653
NaCl 0.90 g 0.864 14.78
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Yeast extract
(Oxoid) 		2.00 g 1.919 -
Resazurin 0.50 mg 4.8e-4 0.002
NaHCO3 2.00 g 1.919 22.848
Distilled water 1000.00 ml - -
1 Mix components except NaHCO3 and bring to a boil for a few seconds. After cooling down under a stream of N2-CO2 (80:20, v/v), add NaHCO3 to the medium. Dispense the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Allow to stand the medium until re-dissolved. Then, add per liter the following solutions (autoclaved or *filter-sterilized):
Trace vitamins
(*) 		10.00 ml - -
Methanol
(v/v, 50%) 		10.00 ml - -
L-Cysteine HCl x H2O
(5%) 		10.00 ml - -
Na2S x 9 H2O
(5%) 		10.00 ml - -
2 Adjust pH of the medium to 6.8-7.0, if necessary.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.9
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.919 22.848
Yeast extract 1.919 -
NH4Cl 0.96 17.942
NaCl 0.864 14.78
K2HPO4 0.278 1.598
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain