Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 120.0 | g | 116.505 | 1993.58 | |
MgCl2 x 6 H2O | 6.0 | g | 5.825 | 28.653 | |
KCl | 1.5 | g | 1.456 | 19.535 | |
Na2SO4 | 1.0 | g | 0.971 | 6.835 | |
NH4Cl | 1.0 | g | 0.971 | 18.151 | |
CaCl2 x 2 H2O | 0.4 | g | 0.388 | 2.642 | |
K2HPO4 | 0.4 | g | 0.388 | 2.23 | |
Trace mineral solution | 10.0 | ml | - | - | |
Resazurin | 0.5 | mg | 4.9e-4 | 0.002 | |
Distilled water | 950.0 | ml | - | - | |
1 Mix components thoroughly, bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream and autoclave under the same gas mixture. After cooling, add per liter the following solutions (autoclaved or *filter-sterilized): | |||||
Glucose
(10%) |
10.0 | ml | - | - | |
Trace vitamins
(*) |
10.0 | ml | - | - | |
NaHCO3
(*, 8%) |
30.0 | ml | - | - | |
2 Aseptically and anaerobically, dispense the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solutions (autoclaved and stored under a N2 atmosphere): | |||||
L-Cysteine HCl x H2O
(5%) |
10.0 | ml | - | - | |
Na2S x 9 H2O
(5%) |
10.0 | ml | - | - | |
3 Readjust pH to 7.5, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Trace minerals | 1000.00 | ml | - | - |
NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 |
Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 |
Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 7.5 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter, Hungate tubes |
Edit in Medium builder |
All strains for this medium: | 2 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 116.515 | 1993.75 |
MgCl2 x 6 H2O | 5.825 | 28.653 |
KCl | 1.456 | 19.535 |
NH4Cl | 0.971 | 18.151 |
Na2SO4 | 0.971 | 6.835 |
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