JCM 1180 . CELLULOLYTIC NATRONOARCHAEA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 128.00 g 4571.43 78224.3
Na2CO3 95.00 g 3392.86 32011.7
NaHCO3 15.00 g 535.714 6377.02
KCl 2.50 g 89.286 1197.66
K2HPO4 1.75 g 62.5 358.832
1 Add components to distilled water and bring volume to 1.0 L. After cooling, add the following solutions (autoclaved or *filter-sterilized):
NH4Cl
(10%)
1.25 ml - -
MgSO4
(1 M)
1.00 ml - -
Trace element solution 1.00 ml - -
Trace vitamins
(*)
5.00 ml - -
Yeast extract
(10%)
0.20 ml - -
Cellobiose
(10%)
20.00 ml - -
Solution occurs in 13 other media (first defined in Medium J1079).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 -
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 Adjust pH to 3.6.
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 4571.43 78224.3
Na2CO3 3392.86 32011.7
NaHCO3 535.714 6377.02
KCl 89.286 1197.66
K2HPO4 62.5 358.832
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature psychrophilic 1 strain