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J1180. CELLULOLYTIC NATRONOARCHAEA MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 128.00 g 4571.43 78221
2 95.00 g 3392.86 32011.7
3 15.00 g 535.714 6377.05
4 2.50 g 89.286 1197.65
5 1.75 g 62.5 358.832
6
(10%)
1.25 ml 0.007 0.128
7
(1 M)
1.00 ml 95 789.24
8 Trace element solution 1.00 ml - -
9 Trace vitamins
(*)
5.00 ml - -
10
(10%)
0.20 ml - -
11
(10%)
20.00 ml - -
Instructions:
  1. Add components to distilled water and bring volume to 1.0 L. After cooling, add the following solutions (autoclaved or *filter-sterilized): #6-11
Solution occurs in 13 other media (first defined in medium J1079 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 17.228
2 2.00 g 2 7.194
3 0.10 g 0.1 -
4 0.03 g 0.03 -
5 0.30 g 0.3 4.852
6 0.20 g 0.2 -
7 0.01 g 0.01 -
8 0.02 g 0.02 -
9 0.03 g 0.03 0.124
10 1000.00 ml 998.207 55408.8
Instructions:
  1. Adjust pH to 3.6.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 4571.43 78221
Na2CO3 3392.86 32011.7
NaHCO3 535.714 6377.05
Distilled water 213.902 11873.3
MgSO4 95 789.24
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