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JCM 1180 . CELLULOLYTIC NATRONOARCHAEA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1180 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 128.00 g 4571.43 78224.3
Na2CO3 95.00 g 3392.86 32011.7
NaHCO3 15.00 g 535.714 6377.02
KCl 2.50 g 89.286 1197.66
K2HPO4 1.75 g 62.5 358.832
1 Add components to distilled water and bring volume to 1.0 L. After cooling, add the following solutions (autoclaved or *filter-sterilized):
NH4Cl
(10%) 		1.25 ml - -
MgSO4
(1 M) 		1.00 ml - -
Trace element solution 1.00 ml - -
Trace vitamins
(*) 		5.00 ml - -
Yeast extract
(10%) 		0.20 ml - -
Cellobiose
(10%) 		20.00 ml - -

Trace element solution #

1000
Solution occurs in 13 other media (first defined in Medium J1079).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 -
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 Adjust pH to 3.6.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 4571.43 78224.3
Na2CO3 3392.86 32011.7
NaHCO3 535.714 6377.02
KCl 89.286 1197.66
K2HPO4 62.5 358.832
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature psychrophilic 1 strain