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JCM 1181 . MODIFIED CELLULOLYTIC HALOARCHAEA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1181 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MDS salt water 833.0 ml - -
Trace element solution 1.0 ml - -
Yeast extract
(BD-Difco) 		0.1 g 0.114 -
1 Add components and bring volume to 958 ml with distilled water. For preparation of solid medium, add 20 g/L agar (if necessary, use washed agar: see Medium No. 574). Autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized) to the medium.
NH4Cl
(1 M) 		5.0 ml - -
Potassium phosphate buffer 2.0 ml - -
Trace vitamins
(*) 		5.0 ml - -
Cellobiose
(0.2 M) 		30.0 ml - -
2 Readjust pH to 7.0 with a sterilized 10% Na2CO3 solution, if necessary.

MDS salt water #

1000
Solution occurs in 5 other media (first defined in Medium J574).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 240 g 240 4106.78
MgCl2 x 6 H2O 30 g 30 147.563
MgSO4 x 7 H2O 35 g 35 142.002
KCl 7 g 7 93.897
CaCl2
(1 M) 		5 ml 0.555 5
1 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.5 with 1 M Tris base.

Trace element solution #

1000
Solution occurs in 13 other media (first defined in Medium J1079).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 -
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 Adjust pH to 3.6.

Potassium phosphate buffer #

100
Solution occurs in 2 other media (first defined in Medium J574).
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4
(1 M) 		83.4 ml 14.526 83.4
KH2PO4
(1 M) 		16.6 ml 2.259 16.6
1 Combine the two solutions and check that pH is close to 7.5. Add an equal volume of distilled water.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 45643.8 781037
MgSO4 x 7 H2O 6656.39 27006.2
MgCl2 x 6 H2O 5705.48 28063.9
KCl 1331.28 17857.5
Yeast extract 0.114 -
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Cultivation metadata from 1 strains BacDive

halophily extremely halophilic 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen aerobe 1 strain
temperature mesophilic 1 strain
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