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JCM 1181 . MODIFIED CELLULOLYTIC HALOARCHAEA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MDS salt water 833.0 ml - -
Trace element solution 1.0 ml - -
Yeast extract
(BD-Difco)
0.1 g 0.114 -
1 Add components and bring volume to 958 ml with distilled water. For preparation of solid medium, add 20 g/L agar (if necessary, use washed agar: see Medium No. 574). Autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized) to the medium.
NH4Cl
(1 M)
5.0 ml - -
Potassium phosphate buffer 2.0 ml - -
Trace vitamins
(*)
5.0 ml - -
Cellobiose
(0.2 M)
30.0 ml - -
2 Readjust pH to 7.0 with a sterilized 10% Na2CO3 solution, if necessary.
Solution occurs in 5 other media (first defined in Medium J574).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 240 g 48000 821355
MgCl2 x 6 H2O 30 g 6000 29512.6
MgSO4 x 7 H2O 35 g 7000 28400.3
KCl 7 g 1400 18779.3
CaCl2
(1 M)
5 ml - -
1 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.5 with 1 M Tris base.
Solution occurs in 13 other media (first defined in Medium J1079).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 -
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 Adjust pH to 3.6.
Solution occurs in 2 other media (first defined in Medium J574).
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4
(1 M)
83.4 ml - -
KH2PO4
(1 M)
16.6 ml - -
1 Combine the two solutions and check that pH is close to 7.5. Add an equal volume of distilled water.
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 45643.8 781037
MgSO4 x 7 H2O 6656.39 27006.2
MgCl2 x 6 H2O 5705.48 28063.9
KCl 1331.28 17857.5
Yeast extract 0.114 -
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