Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
MDS salt water | 833.0 | ml | ||
|
Trace element solution | 1.0 | ml | ||
|
Yeast extract
(BD-Difco) |
0.1 | g | ||
1 Add components and bring volume to 958 ml with distilled water. For preparation of solid medium, add 20 g/L agar (if necessary, use washed agar: see Medium No. 574). Autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized) to the medium. | |||||
|
NH4Cl
(1 M) |
5.0 | ml | ||
|
Potassium phosphate buffer | 2.0 | ml | ||
|
Trace vitamins
(*) |
5.0 | ml | ||
|
Cellobiose
(0.2 M) |
30.0 | ml | ||
2 Readjust pH to 7.0 with a sterilized 10% Na2CO3 solution, if necessary. |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
NaCl | 240 | g | ||
|
MgCl2 x 6 H2O | 30 | g | ||
|
MgSO4 x 7 H2O | 35 | g | ||
|
KCl | 7 | g | ||
|
CaCl2
(1 M) |
5 | ml | ||
1 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.5 with 1 M Tris base. |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
EDTA | 5.00 | g | ||
|
FeSO4 x 7 H2O | 2.00 | g | ||
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
MnCl2 x 4 H2O | 0.03 | g | ||
|
H3BO3 | 0.30 | g | ||
|
CoCl2 x 6 H2O | 0.20 | g | ||
|
CuCl2 x 2 H2O | 0.01 | g | ||
|
NiCl2 x 6 H2O | 0.02 | g | ||
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
Distilled water | 1000.00 | ml | ||
1 Adjust pH to 3.6. |
Compound | Amount | Unit | |
---|---|---|---|
|
Biotin | 2.0 | mg |
|
Folic acid | 2.0 | mg |
|
Pyridoxine hydrochloride | 10.0 | mg |
|
Thiamine HCl | 5.0 | mg |
|
Riboflavin | 5.0 | mg |
|
Nicotinic acid | 5.0 | mg |
|
Calcium pantothenate | 5.0 | mg |
|
Vitamin B12 | 0.1 | mg |
|
p-Aminobenzoic acid | 5.0 | mg |
|
Lipoic acid | 5.0 | mg |
|
Distilled water | 1000.0 | ml |