J1182
1182 .
FERROUS IRON YEAST EXTRACT MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1182 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 0.50 | g | 0.485 | 1.968 | |
| (NH4)2SO4 | 0.45 | g | 0.436 | 3.303 | |
| Na2SO4 | 0.15 | g | 0.145 | 1.024 | |
| KH2PO4 | 0.05 | g | 0.048 | 0.356 | |
| KCl | 0.05 | g | 0.048 | 0.651 | |
| Ca(NO3)2 x 4 H2O | 0.01 | g | 0.01 | 0.041 | |
| Trace element solution | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH 2.0 with H2SO4 and autoclave. After cooling, aseptically add the following solutions (autoclave or *filer-sterilized): | |||||
|
FeSO4
(pH 2.0, *, 1 M) |
10.00 | ml | - | - | |
|
Yeast extract
(1.0%) |
20.00 | ml | - | - | |
Solution occurs in 1
other media (first defined in Medium J1182).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| ZnSO4 x 7 H2O | 10.0 | g | 10 | 34.775 |
| CuSO4 x 5 H2O | 1.0 | g | 1 | 4.005 |
| MnSO4 x n H2O | 1.0 | g | 1 | - |
| CoSO4 x 7 H2O | 1.0 | g | 1 | 6.452 |
| Cr2(SO4)3 x n H2O | 0.5 | g | 0.5 | - |
| H3BO3 | 0.6 | g | 0.6 | 9.702 |
| Na2MoO4 x 2 H2O | 0.5 | g | 0.5 | 2.067 |
| NiSO4 x 6 H2O | 1.0 | g | 1 | 6.462 |
| Na2SeO4 | 1.0 | g | 1 | 5.292 |
| Na2WO4 x 2 H2O | 1.0 | g | 1 | 3.032 |
| NaVO3 | 0.1 | g | 0.1 | 0.82 |
|
H2SO4
(0.01 M) |
1000.0 | ml | - | - |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| MgSO4 x 7 H2O | 0.485 | 1.968 |
| (NH4)2SO4 | 0.436 | 3.303 |
| Na2SO4 | 0.145 | 1.024 |
| KCl | 0.048 | 0.651 |
| KH2PO4 | 0.048 | 0.356 |
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