J1190
1190 .
VALLITALEA 70B-A MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1190 #
ml
Equipment needed: Autoclave
Anaerobic workbench
| 1 Mix components thoroughly, adjust pH to 7.9 with NaOH and autoclave under a N2 atmosphere. After cooling, add the following solutions: | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| HEPES | 4.7 | g | 4.649 | 19.508 | |
| NH4Cl | 0.5 | g | 0.495 | 9.246 | |
| KH2PO4 | 0.3 | g | 0.297 | 2.181 | |
| NaCl | 25.0 | g | 24.728 | 423.135 | |
| KCl | 0.3 | g | 0.297 | 3.98 | |
| Yeast extract | 0.2 | g | 0.198 | - | |
| Trace element solution | 1.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 950.0 | ml | - | - | |
|
Glucose
(1 M) |
20.0 | ml | - | - | |
|
MgCl2 x 6 H2O
(5%) |
20.0 | ml | - | - | |
|
CaCl2 x 2 H2O
(1%) |
10.0 | ml | - | - | |
| 2 Aseptically and anaerobically distribute the medium into culture vessels under a N2 gas stream and seal with butyl rubber stoppers. Prior inoculation, add per liter the following solution from an anaerobic stock (autoclaved and stored under a N2 atmosphere): | |||||
|
Na2S x 9 H2O
(5%) |
10.0 | ml | - | - | |
Solution occurs in 38
other media (first defined in Medium J439).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(25%) |
12.5 | ml | - | - |
| FeSO4 x 7 H2O | 2.1 | g | 2.1 | 7.554 |
| H3BO3 | 30.0 | mg | 0.03 | 0.485 |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | - |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 |
| ZnSO4 x 7 H2O | 144.0 | mg | 0.144 | 0.501 |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
| Distilled water | 987.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.9 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 24.728 | 423.135 |
| HEPES | 4.649 | 19.508 |
| NH4Cl | 0.495 | 9.246 |
| KCl | 0.297 | 3.98 |
| KH2PO4 | 0.297 | 2.181 |
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