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JCM 1199 . MINERAL MEDIUM (BRUNNER) SUPPLEMENTED WITH TOLUENE

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1199 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.50 g 0.249 1.883
MgSO4 x 7 H2O 0.20 g 0.1 0.404
CaCl2 x 2 H2O 0.05 g 0.025 0.169
Trace element solution SL-4 10.00 ml - -
Agar 15.00 g 7.463 22.189
Distilled water 900.00 ml - -
1 Adjust pH to 6.9. After autoclaving, aseptically add the phosphates solution to the medium. After inoculation, put several drops of toluene inside of lid of petri dishes.
Phosphates solution 100.00 ml - -
2 Phosphates solution:
Na2HPO4 x 2 H2O 2.44 g 1.214 8.551
KH2PO4 1.52 g 0.756 5.557
Distilled water 1000.00 ml - -
3 Adjust pH to 6.9.

Trace element solution SL-4 #

1000
Solution occurs in 21 other media (first defined in Medium J340).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.5 g 0.5 1.711
FeSO4 x 7 H2O 0.2 g 0.2 0.719
Trace element solution SL-6 100.0 ml - -
Distilled water 900.0 ml - -

SL-6 trace element solution #

1000
Solution occurs in 10 other media (first defined in Medium J167).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 Adjust pH to 3.6.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.9
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 7.463 22.189
Na2HPO4 x 2 H2O 1.214 8.551
KH2PO4 0.756 5.557
(NH4)2SO4 0.249 1.883
MgSO4 x 7 H2O 0.1 0.404
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen aerobe 1 strain
temperature mesophilic 1 strain