Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MgCl2 x 6 H2O | 0.93 | g | 0.93 | 4.574 | |
KCl | 1.00 | g | 1 | 13.414 | |
CaCl2 x 2 H2O | 0.12 | g | 0.12 | 0.816 | |
NaNO3 | 0.17 | g | 0.17 | 2 | |
Distilled water | 992.00 | ml | - | - | |
1 Mix components and autoclave. For preparation of solid medium, add 15.0 g/L agar before autoclaving. After cooling to 55C, add the following solutions (autoclaved or *filter-sterilized) to the medium: | |||||
HEPES buffer
(pH 7.0, 2 M) |
5.00 | ml | - | - | |
1 M Phosphate buffer
(1 M) |
0.15 | ml | - | - | |
FeCl2 solution | 1.00 | ml | - | - | |
Trace element solution | 1.00 | ml | - | - | |
Vitamin solution
(*) |
1.00 | ml | - | - | |
2 Distribute the culture medium into culture tubes (leave > 80% volume of a gas phase)and seal with butyl rubber stoppers. Finally, add methane to make a concentration of 50% in the gas phase (by volume). |
1 Mix 28 ml of 1 M KH2PO4 and 78 ml of 1 M Na2HPO4 to prepare 100 ml of 1 M phosphate buffer. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Vitamin B12 | 50 | mg | 0.05 | 0.037 |
p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
Biotin | 10 | mg | 0.01 | 0.041 |
Nicotinic acid | 100 | mg | 0.1 | 0.812 |
DL-Calcium pantothenate | 25 | mg | 0.025 | 0.052 |
Pyridoxine hydrochloride | 250 | mg | 0.25 | - |
Thiamine HCl | 50 | mg | 0.05 | - |
Distilled water | 1000 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Medium type: | Defined medium |
Final pH: | n.d. |
Equipment needed: | Autoclave, Filter |
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