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J1204. SPOROMUSA ACIDOVORANS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.50 g 0.492 9.191
2 0.50 g 0.492 1.995
3 0.25 g 0.246 1.672
4 2.25 g 2.212 37.856
5 FeCl2 solution 1.00 ml - -
6 Trace element solution 1.00 ml - -
7 Se/W solution 1.00 ml - -
8
(in 0.1 N H2SO4, 0.1%)
2.00 ml - -
9 2.00 g 1.967 -
10
(BD-Difco)
2.00 g 1.967 -
11 0.50 mg 4.9e-4 0.002
12 830.00 ml 814.663 45220.6
13 0.35 g 0.344 1.976
14 0.23 g 0.226 1.662
15 100.00 ml 98.152 5448.26
16 Trace vitamins 10.00 ml - -
17
(w/v, 40%)
10.00 ml - -
18
(8%)
50.00 ml 8.492 101.083
19
(5%)
6.00 ml - -
20
(5%)
6.00 ml - -
Instructions:
  1. Solution A:
  2. Autoclave under a N2 gas atmosphere.
  3. Solution B: #13-15
  4. Autoclave under a N2 gas atmosphere.
  5. After autoclaving, combine solutions A and B, then add the following solutions (filter-sterilized): #16-18
  6. To complete the medium, aseptically and anaerobically distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Prior to use, add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere): #19-20
  7. Adjust pH to 6.5-7.0, if necessary.
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 4 other media (first defined in medium J317 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4 mg 0.004 0.023
2 4 mg 0.004 -
3 1000 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 925.565 51376.6
NaHCO3 8.492 101.083
NaCl 2.212 37.856
Yeast extract 1.967 -
Casitone 1.967 -
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