MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 1204 . SPOROMUSA ACIDOVORANS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1204 #

ml
Equipment needed: Hungate tubes Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.50 g 0.492 9.191
MgSO4 x 7 H2O 0.50 g 0.492 1.995
CaCl2 x 2 H2O 0.25 g 0.246 1.672
NaCl 2.25 g 2.212 37.857
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Se/W solution 1.00 ml - -
FeSO4 x 7 H2O
(in 0.1 N H2SO4, 0.1%) 		2.00 ml - -
Yeast extract 2.00 g 1.967 -
Casitone
(BD-Difco) 		2.00 g 1.967 -
Resazurin 0.50 mg 4.9e-4 0.002
Distilled water 830.00 ml - -
1 Solution B:
K2HPO4 0.35 g 0.344 1.976
KH2PO4 0.23 g 0.226 1.662
Distilled water 100.00 ml - -
2 After autoclaving, combine solutions A and B, then add the following solutions (filter-sterilized):
Trace vitamins 10.00 ml - -
D-Fructose
(w/v, 40%) 		10.00 ml - -
NaHCO3
(8%) 		50.00 ml - -
3 To complete the medium, aseptically and anaerobically distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Prior to use, add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere):
L-Cysteine HCl x H2O
(5%) 		6.00 ml - -
Na2S x 9 H2O
(5%) 		6.00 ml - -
4 Solution A:
5 Autoclave under a N2 gas atmosphere.
6 Autoclave under a N2 gas atmosphere.
7 Adjust pH to 6.5-7.0, if necessary.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Se/W solution #

1000
Solution occurs in 4 other media (first defined in Medium J317).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 4 mg 0.004 -
Na2WO4 x 2 H2O 4 mg 0.004 0.012
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 2.212 37.857
Casitone 1.967 -
Yeast extract 1.967 -
NH4Cl 0.492 9.191
MgSO4 x 7 H2O 0.492 1.995
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain