Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 70.0 | g | 67.961 | 1162.92 | |
Na2CO3 | 40.0 | g | 38.835 | 366.409 | |
Distilled water | 500.0 | ml | - | - | |
1 Solution B: | |||||
K2HPO4 | 6.3 | g | 6.117 | 35.117 | |
Basal salts solution
(see below) |
10.0 | ml | - | - | |
Trace minerals | 10.0 | ml | - | - | |
Distilled water | 480.0 | ml | - | - | |
2 Autoclave solutions A and B, separately, under a N2 gas atmosphere. After cooling, combine both solutions and add the following solution (autoclaved and stored under N2): | |||||
Yeast extract
(10%) |
10.0 | ml | - | - | |
3 Aseptically and anaerobically, distribute the medium into culture vessels (e.g., 10 ml in Balch type tubes) under a N2 gas stream and seal with butyl rubber stoppers. Finally, add per liter the following solutions (autoclaved and stored under N2): | |||||
L-Cysteine HCl x H2O
(5%) |
10.0 | ml | - | - | |
Na2S x 9 H2O
(5%) |
10.0 | ml | - | - | |
4 Solution A: |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NH4NO3 | 50.0 | mg | 0.05 | - |
MgCl2 x 6 H2O | 8.5 | mg | 0.009 | 0.042 |
SiO2 | 7.5 | mg | 0.008 | 0.125 |
MnSO4 x n H2O | 4.5 | mg | 0.005 | - |
CaCl2 x 2 H2O | 4.2 | mg | 0.004 | 0.029 |
Methylene blue | 4.0 | mg | 0.004 | 0.013 |
FeSO4 x 7 H2O | 1.8 | mg | 0.002 | 0.006 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. |
Last modified: | 29.10.21 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 67.971 | 1163.09 |
Na2CO3 | 38.835 | 366.409 |
K2HPO4 | 6.117 | 35.117 |
MgSO4 x 7 H2O | 0.029 | 0.118 |
Nitrilotriacetic acid | 0.015 | 0.076 |
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