J1211
1211 .
METHANOCALDOCOCCUS LEIGHII MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1211 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 22.000 | g | 10.837 | 185.446 | |
| MgCl2 x 6 H2O | 2.700 | g | 1.33 | 6.542 | |
| MgSO4 x 7 H2O | 3.400 | g | 1.675 | 6.795 | |
| NH4Cl | 0.500 | g | 0.246 | 4.605 | |
| KCl | 0.330 | g | 0.163 | 2.181 | |
| K2HPO4 | 0.140 | g | 0.069 | 0.396 | |
| CaCl2 x 2 H2O | 0.140 | g | 0.069 | 0.469 | |
| FeSO4 x 7 H2O | 9.500 | mg | 0.005 | 0.017 | |
|
Trace mineral solution
(see below) |
10.000 | ml | - | - | |
| Trace vitamins | 10.000 | ml | - | - | |
| Sodium acetate x 3 H2O | 1.400 | g | 0.69 | - | |
| PIPES | 15.120 | g | 7.448 | 24.631 | |
| NaHCO3 | 1.000 | g | 0.493 | 5.864 | |
| Resazurin | 1.000 | mg | 4.9e-4 | 0.002 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Mix components, except NaHCO3, and adjust pH to 6.0. Bring to a boil for 5-10 sec, cool down under a stream of H2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g., 5 ml in Balch type tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solution (autoclaved and stored under N2): | |||||
|
Na2S x 9 H2O
(5%) |
10.000 | ml | - | - | |
| 2 Trace mineral solution: | |||||
| Trisodium citrate x 2 H2O | 2.100 | g | 1.034 | - | |
| MgSO4 x 7 H2O | 3.000 | g | 1.478 | 5.996 | |
| MnSO4 x n H2O | 0.500 | g | 0.246 | - | |
| CoSO4 x 7 H2O | 0.100 | g | 0.049 | 0.318 | |
| ZnSO4 x 7 H2O | 0.100 | g | 0.049 | 0.171 | |
| Na2MoO4 x 2 H2O | 0.100 | g | 0.049 | 0.204 | |
| NiCl2 x 6 H2O | 0.025 | g | 0.012 | 0.095 | |
| Na2SeO3 | 0.020 | g | 0.01 | - | |
| CuSO4 x 5 H2O | 0.010 | g | 0.005 | 0.02 | |
| AlK(SO4)2 | 0.010 | g | 0.005 | 0.019 | |
| H3BO3 | 0.010 | g | 0.005 | 0.08 | |
| VOSO4 x n H2O | 0.010 | g | 0.005 | - | |
| Na2WO4 x 2 H2O | 3.300 | mg | 0.002 | 0.005 | |
| Distilled water | 1000.000 | ml | - | - | |
| 3 Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20, v/v). | |||||
| 4 Comment: For growth by nitrogen-fixation, remove NH4Cl from the medium and cultivate under a H2-N2-CO2 (60:20:20, v/v/v, 200 kPa) gas mixture. | |||||
| 5 Dissolve trisodium citrate and adjust pH to 6.5. Then proceed to add minerals. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | 6.5 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |