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J1211. METHANOCALDOCOCCUS LEIGHII MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 22.000 g 10.837 185.438
2 2.700 g 1.33 6.542
3 3.400 g 1.675 6.795
4 0.500 g 0.246 4.605
5 0.330 g 0.163 2.181
6 0.140 g 0.069 0.396
7 0.140 g 0.069 0.469
8 9.500 mg 0.005 0.017
9
(see below)
10.000 ml - -
10 Trace vitamins 10.000 ml - -
11 1.400 g 0.69 -
12 15.120 g 7.448 -
13 1.000 g 0.493 5.864
14 1.000 mg 4.9e-4 0.002
15 1000.000 ml 491.728 27295
16
(5%)
10.000 ml - -
17 2.100 g 1.034 -
18 3.000 g 1.478 5.996
19 0.500 g 0.246 -
20 0.100 g 0.049 -
21 0.100 g 0.049 -
22 0.100 g 0.049 0.204
23 0.025 g 0.012 -
24 0.020 g 0.01 0.057
25 0.010 g 0.005 -
26 0.010 g 0.005 0.019
27 0.010 g 0.005 0.08
28 0.010 g 0.005 -
29 3.300 mg 0.002 -
30 1000.000 ml 491.728 27295
Instructions:
  1. Mix components, except NaHCO3, and adjust pH to 6.0. Bring to a boil for 5-10 sec, cool down under a stream of H2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g., 5 ml in Balch type tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solution (autoclaved and stored under N2): #16
  2. Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20, v/v).
  3. Comment: For growth by nitrogen-fixation, remove NH4Cl from the medium and cultivate under a H2-N2-CO2 (60:20:20, v/v/v, 200 kPa) gas mixture.
  4. Trace mineral solution: #17-30
  5. Dissolve trisodium citrate and adjust pH to 6.5. Then proceed to add minerals.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 988.373 54862.9
NaCl 10.837 185.438
PIPES 7.448 -
MgSO4 x 7H2O 3.153 12.791
MgCl2 x 6H2O 1.33 6.542
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