J1212
1212 .
MODIFIED MAKKAR'S MEDIUM C
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1212 #
ml
Equipment needed: Anaerobic workbench
Autoclave
| 1 Mix components, except L-cysteine x HCl x H2O, and heat the medium to 60C with stirring. Bubble the medium with O2-free CO2 vigorously, then add L-cysteine x HCl x H2O to reduce the medium. Dispense the medium into culture vessels (e.g., 10 ml in Balch type tubes) under a stream of CO2, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add per liter the following solution (autoclaved and stored under a N2 atmosphere): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Solution I | 150.0 | ml | - | - | |
| Solution II | 150.0 | ml | - | - | |
|
Rumen fluid
, clarified
(see Medium No. 266) |
50.0 | ml | - | - | |
| Yeast extract | 2.5 | g | 2.451 | - | |
|
Casitone
(BD-Difco) |
10.0 | g | 9.804 | - | |
| NaHCO3 | 6.0 | g | 5.882 | 70.022 | |
| L-Cysteine HCl x H2O | 1.0 | g | 0.98 | - | |
| Resazurin | 1.0 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 650.0 | ml | - | - | |
|
Cellobiose
(0.2 M) |
20.0 | ml | - | - | |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| K2HPO4 | 3 | g | 3 | 17.224 |
| Distilled water | 1000 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| KH2PO4 | 3.0 | g | 3 | 22.045 |
| (NH4)2SO4 | 6.0 | g | 6 | 45.406 |
| NaCl | 6.0 | g | 6 | 102.669 |
| MgSO4 x 7 H2O | 0.6 | g | 0.6 | 2.434 |
| CaCl2 x 2 H2O | 0.6 | g | 0.6 | 4.081 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |