Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Trypticase peptone | 5.0 | g | 4.995 | - | |
Peptone | 5.0 | g | 4.995 | - | |
Yeast extract | 10.0 | g | 9.99 | - | |
Beef extract | 5.0 | g | 4.995 | - | |
Glucose | 5.0 | g | 4.995 | 27.725 | |
KH2PO4 | 2.0 | g | 1.998 | 14.682 | |
Tween 80 | 1.0 | ml | - | - | |
Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
Salt solution | 40.0 | ml | - | - | |
Distilled water | 890.0 | ml | - | - | |
1 Mix components thoroughly, bring to a boil and cool down under a stream of O2-free CO2. While gassing, add the following solutions and adjust pH to 7.2: | |||||
Rumen fluid
, clarified
(see Medium No. 266) |
50.0 | ml | - | - | |
L-Cysteine HCl x H2O
(5%) |
10.0 | ml | - | - | |
Hemin solution | 10.0 | ml | - | - | |
Vitamin K1 solution | 0.2 | ml | - | - | |
2 Distribute the medium into culture vessels (e.g., 10 ml in Balch type tubes) under N2, seal with butyl rubber stoppers and autoclave. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Vitamin K1 | 0.1 | ml | - | - | |
Ethanol
(95 %) |
20.0 | ml | 950 | 20620.8 | |
1 Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 |
MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 |
K2HPO4 | 1.00 | g | 1 | 5.741 |
KH2PO4 | 1.00 | g | 1 | 7.348 |
NaHCO3 | 10.00 | g | 10 | 119.038 |
NaCl | 2.00 | g | 2 | 34.223 |
Distilled water | 1000.00 | ml | - | - |
1 Dissolve 50 mg hemin first in 1 ml of 1 N NaOH. Then adjust volume to 100 ml. |
Last modified: | 22.02.22 |
Source: | JCM |
Medium type: | Complex medium |
Final pH: | 7.2 |
Equipment needed: | Autoclave, Filter |
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