Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Major salts solution | 100 | ml | - | - | |
NaCl | 10 | g | 9.901 | 169.421 | |
Peptone | 1 | g | 0.99 | - | |
Yeast extract | 1 | g | 0.99 | - | |
HEPES | 1 | g | 0.99 | 4.155 | |
Trace minerals | 20 | ml | - | - | |
Trace vitamins | 20 | ml | - | - | |
Distilled water | 860 | ml | - | - | |
1 Mix components thoroughly, adjust pH to 7.2 and autoclave under a H2-CO2 (80:20, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solution (autoclaved and stored under N2): | |||||
Na2S x 9 H2O
(5%) |
10 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
KCl | 0.120 | g | 0.12 | 1.61 |
MgSO4 x 7 H2O | 0.300 | g | 0.3 | 1.217 |
CaSO4 x 2 H2O | 0.076 | g | 0.076 | 0.441 |
Na2SO4 | 0.400 | g | 0.4 | 2.816 |
CaCl2 x 2 H2O | 0.024 | g | 0.024 | 0.163 |
NH4Cl | 0.250 | g | 0.25 | 4.674 |
(NH4)2SO4 | 0.320 | g | 0.32 | 2.422 |
KH2PO4 | 0.450 | g | 0.45 | 3.307 |
NaF | 0.060 | g | 0.06 | 1.429 |
LiCl | 0.020 | g | 0.02 | 0.472 |
Distilled water | 1000.000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.2 |
Equipment needed: | Anaerobic workbench, Autoclave |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 9.921 | 169.76 |
HEPES | 0.99 | 4.155 |
Peptone | 0.99 | - |
Yeast extract | 0.99 | - |
MgSO4 x 7 H2O | 0.089 | 0.362 |
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