1227 .
ZESTOSPHAERA MEDIUM
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Equipment needed:
Autoclave
Final pH:
6.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Modified Brock's salt base solution | 1000.0000 | ml | ||
|
|
Na2S2O3 x 5 H2O | 2.0000 | g | ||
|
|
NaOH | 0.1600 | g | ||
|
|
Resazurin | 1.0000 | mg | ||
|
|
Trace mineral solution | 10.0000 | ml | ||
|
|
Distilled water | 1000.0000 | ml | ||
| 1 Heat the medium to boiling while bubbling with a N2-CO2 (4:1, v/v) gas mixture. After boiling, cool the medium in a water bath. Continue bubbling with the same gas mixture. While the medium is still warm, add the following: | |||||
|
|
Yeast extract
(Oxoid) |
1.0000 | g | ||
|
|
Tryptone | 1.0000 | g | ||
|
|
Casamino acids
(BD-Difco) |
0.5000 | g | ||
|
|
L-Cysteine HCl x H2O | 0.0625 | g | ||
| 2 After the medium is cooled to room temperature, adjust pH to 6.0 - 6.1. Dispense the medium into culture vessels (e.g., 10 ml in Balch type tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. | |||||
Modified Brock's salt base solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
(NH4)2SO4 | 1.30 | g |
|
|
KH2PO4 | 0.28 | g |
|
|
MgSO4 x 7 H2O | 0.25 | g |
|
|
CaCl2 x 2 H2O | 0.07 | g |
|
|
FeCl3 x 6 H2O | 2.00 | mg |
|
|
MnCl2 x 4 H2O | 1.80 | mg |
|
|
Na2B4O7 x 10 H2O | 4.50 | mg |
|
|
ZnSO4 x 7 H2O | 0.22 | mg |
|
|
CuCl2 x 2 H2O | 0.05 | mg |
|
|
Na2MoO4 x 2 H2O | 0.03 | mg |
|
|
VOSO4 x n H2O | 0.03 | mg |
|
|
CoSO4 x 7 H2O | 0.01 | mg |
|
|
Distilled water | 1000.00 | ml |
Trace mineral solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Na2-EDTA | 0.50 | g |
|
|
CoCl2 x 6 H2O | 0.15 | g |
|
|
MnCl2 x 4 H2O | 0.10 | g |
|
|
FeSO4 x 7 H2O | 0.10 | g |
|
|
ZnCl2 | 0.10 | g |
|
|
AlCl3 x 6 H2O | 0.04 | g |
|
|
Na2WO2 x 2 H2O | 0.03 | g |
|
|
CuCl2 x 2 H2O | 0.02 | g |
|
|
NiSO4 x 6 H2O | 0.02 | g |
|
|
H2SeO3 | 0.01 | g |
|
|
H3BO3 | 0.01 | g |
|
|
Na2MoO4 x 2 H2O | 0.01 | g |
|
|
Distilled water | 1000.00 | ml |