Loading ...

J1228. ANAEROBIC ALKALINE GLUCOSE MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 20.00 g 19.474 333.22
2 50.00 g 48.686 459.349
3 60.00 g 58.423 695.452
4 900.00 ml 874.768 48556.9
5 0.10 g 0.097 0.479
6 0.50 g 0.487 9.102
7 0.20 g 0.195 1.431
8 0.05 g 0.049 -
9 3.00 g 2.921 16.214
10 Trace element solution 1.00 ml - -
11 100.00 ml 97.196 5395.21
12 Trace vitamins 10.00 ml - -
13
(10%)
10.00 ml - -
14
(5%)
6.00 ml - -
Instructions:
  1. Solution A:
  2. Mix components thoroughly and autoclave under a N2 gas atmosphere.
  3. Solution B: #5-11
  4. Mix components thoroughly and autoclave under a N2 gas atmosphere.
  5. Aseptically and anaerobically combine Solutions A and B. Check final pH of the medium to be around 9.2. Add the following solution into the medium (filter-sterilized and stored under a N2 atmosphere): #12
  6. Comment: For JCM 32929, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere): #13-14
Solution occurs in 2 other media (first defined in medium J899 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 784 mg 0.784 -
2 238 mg 0.238 -
3 395 mg 0.395 -
4 24 mg 0.024 0.099
5 33 mg 0.033 -
6 144 mg 0.144 -
7 2 mg 0.002 -
8 94 mg 0.094 0.498
9 6 mg 0.006 0.097
10 99 mg 0.099 -
11 1000 ml 998.207 55408.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 982.656 54545.6
NaHCO3 58.423 695.452
Na2CO3 48.686 459.349
NaCl 19.474 333.22
Glucose 2.921 16.214
Show more

Similar media

1696. MEDIUM FOR Z-1701, Z-1702 86 %
J1289. NATRONIELLA MEDIUM FOR ANB-PHB2 83 %
1505. PROTEINIVORAX MEDIUM 82 %
784. NATRONIELLA MEDIUM 82 %
931. ALCALIPHILIC AMPHIBACILLUS STRAINS MEDIUM 81 %

Latest comments

No comments for this medium.