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JCM 1228 . ANAEROBIC ALKALINE GLUCOSE MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1228 #

ml
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 19.474 333.234
Na2CO3 50.00 g 48.686 459.349
NaHCO3 60.00 g 58.423 695.449
Distilled water 900.00 ml - -
1 Solution B:
MgCl2 x 6 H2O 0.10 g 0.097 0.479
NH4Cl 0.50 g 0.487 9.102
KH2PO4 0.20 g 0.195 1.431
Yeast extract 0.05 g 0.049 -
Glucose 3.00 g 2.921 16.214
Trace element solution 1.00 ml - -
Distilled water 100.00 ml - -
2 Aseptically and anaerobically combine Solutions A and B. Check final pH of the medium to be around 9.2. Add the following solution into the medium (filter-sterilized and stored under a N2 atmosphere):
Trace vitamins 10.00 ml - -
3 Comment: For JCM 32929, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
Peptone
(10%) 		10.00 ml - -
Na2S x 9 H2O
(5%) 		6.00 ml - -
4 Solution A:
5 Mix components thoroughly and autoclave under a N2 gas atmosphere.
6 Mix components thoroughly and autoclave under a N2 gas atmosphere.

Trace element solution #

1000
Solution occurs in 2 other media (first defined in Medium J899).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe(NH4)2(SO4)2 x 6 H2O 784 mg 0.784 -
CoCl2 x 6 H2O 238 mg 0.238 1.833
(NH4)2Ni(SO4)2 x 6 H2O 395 mg 0.395 1
Na2MoO4 x 2 H2O 24 mg 0.024 0.099
Na2WO4 x 2 H2O 33 mg 0.033 0.1
ZnSO4 x 7 H2O 144 mg 0.144 0.501
CuCl2 x 2 H2O 2 mg 0.002 0.012
Na2SeO4 94 mg 0.094 0.497
H3BO3 6 mg 0.006 0.097
MnCl2 x 4 H2O 99 mg 0.099 -
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Complex medium
Final pH: 9.2
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 58.423 695.449
Na2CO3 48.686 459.349
NaCl 19.474 333.234
Glucose 2.921 16.214
NH4Cl 0.487 9.102
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