J1229
1229 .
THERMODESULFOBACTERIUM AUXILIATOTRIS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1229 #
ml
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 3.000 | g | 2.913 | 49.84 | |
| KCl | 0.150 | g | 0.146 | 1.953 | |
| Na2SO4 | 0.300 | g | 0.291 | 2.051 | |
| NH4Cl | 0.107 | g | 0.104 | 1.942 | |
| NaH2PO4 | 1.380 | g | 1.34 | 11.167 | |
| Mineral solution | 5.000 | ml | - | - | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 7.0 with NaOH. Distribute the medium into culture vessels (e.g., 10.0 ml in 25 ml serum bottles) under a stream of N2, seal with butyl rubber stoppers and autoclave. After cooling, add 80% headspace volume of filter-sterilized H2 gas into the culture vessels. Aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized and stored anaerobically): | |||||
|
Solution A
(see below) |
10.000 | ml | - | - | |
|
Sodium acetate
(1.0 M) |
1.000 | ml | - | - | |
|
Sodium Thiosulfate
(1.0 M) |
1.000 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
2.000 | ml | - | - | |
|
NaHCO3
(*, 8%) |
1.000 | ml | - | - | |
| 2 Solution A: | |||||
| MgSO4 x 7 H2O | 0.123 | g | 0.119 | 0.484 | |
| CaCl2 x 2 H2O | 0.015 | g | 0.015 | 0.099 | |
| Distilled water | 10.000 | ml | - | - | |
Solution occurs in 5
other media (first defined in Medium J976).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 3.7 | g | 3.7 | 12.661 | |
| FeSO4 x 7 H2O | 1.1 | g | 1.1 | 3.957 | |
| MnCl2 x 4 H2O | 98.4 | mg | 0.098 | - | |
| ZnSO4 x 7 H2O | 28.8 | mg | 0.029 | 0.1 | |
| CoCl2 x 6 H2O | 23.8 | mg | 0.024 | 0.183 | |
| CuCl2 x 2 H2O | 17.2 | mg | 0.017 | 0.101 | |
| Na2MoO4 x 2 H2O | 24.2 | mg | 0.024 | 0.1 | |
| H3BO3 | 31.0 | mg | 0.031 | 0.501 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 6.0 with KOH. | |||||
| 2 Comment: Consistent growth in serum tubes requires pretreatment of butyl rubber stoppers by boiling in 1% sodium sulfide solution (1 hr) followed by boiling in distilled H2O (1 hr, twice). | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | 7.0 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |