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JCM 1237 . TREPONEMA THERMOPHILUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1237 #

ml
Equipment needed: Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Meat peptone
(BD Bacto) 		5.00 g 4.941 -
Tryptone
(BD Bacto) 		5.00 g 4.941 69.509
Yeast extract 10.00 g 9.881 -
KH2PO4 0.30 g 0.296 2.178
NaCl 0.60 g 0.593 10.145
MgCl2 x 6 H2O 0.10 g 0.099 0.486
CaCl2 x 2 H2O 0.08 g 0.079 0.538
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Trace vitamins 10.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 -
L-Cysteine HCl x H2O 0.25 g 0.247 -
Xylan 1.00 g 0.988 -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before inoculation, add cysteine and xylan from a sterile anoxic stock solution prepared under 100% N2 gas and adjust pH of complete medium to 7.0.

Trace element solution SL-10 #

1000
Solution occurs in 20 other media (first defined in Medium J433).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%, 7.7 M) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 9.881 -
Tryptone 4.941 69.509
Meat peptone 4.941 -
Xylan 0.988 -
NaCl 0.593 10.145
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