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JCM 1238 . TANNER MOD2 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1238 #

ml
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 2.00 g 1.919 32.844
NH4Cl 0.25 g 0.24 4.485
KCl 0.25 g 0.24 3.218
KH2PO4 0.25 g 0.24 1.763
MgSO4 x 7 H2O 0.50 g 0.48 1.947
CaCl2 x 2 H2O 0.10 g 0.096 0.653
MOPS 2.09 g 2.006 9.585
Yeast extract 0.50 g 0.48 -
SL-9 mineral solution 10.00 ml - -
L-Cysteine HCl x H2O 0.50 g 0.48 -
Resazurin 0.50 mg 4.8e-4 0.002
Distilled water 990.00 ml - -
1 Mix components thoroughly and adjust pH 7.0. Dispense the medium into culture vessels under a N2 gas atmosphere, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from sterile anaerobic stocks (filter-sterilized):
Fructose
(1 M) 		30.00 ml - -
Trace vitamins 10.00 ml - -
Vitamin B12 solution 0.05 g/L 1.00 ml - -
Coenzyme M
(0.01%) 		1.00 ml - -

SL-9 mineral solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 2.0 g 2 10.464
MnSO4 x n H2O 1.0 g 1 -
Fe(NH4)2(SO4)2 x 6 H2O 0.8 g 0.8 -
CoCl2 x 6 H2O 0.2 g 0.2 1.54
ZnSO4 x 7 H2O 1.0 mg 1.0e-3 0.003
CuCl2 x 2 H2O 20.0 mg 0.02 0.117
NiCl2 x 6 H2O 20.0 mg 0.02 0.154
Na2MoO4 x 2 H2O 20.0 mg 0.02 0.083
Na2SeO4 20.0 mg 0.02 0.106
Na2WO4 x 2 H2O 20.0 mg 0.02 0.061
Distilled water 1000.0 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Vitamin B12 solution 0.05 g/L #

100
Solution occurs in 18 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 5 mg 0.05 0.037
Distilled water 100 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MOPS 2.006 9.585
NaCl 1.919 32.844
MgSO4 x 7 H2O 0.48 1.947
L-Cysteine HCl x H2O 0.48 -
Yeast extract 0.48 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain