J1242
1242 .
ANAEROBACULUM MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1242 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trypticase peptone | 5.0 | g | 4.873 | - | |
| Peptone | 5.0 | g | 4.873 | - | |
| Yeast extract | 10.0 | g | 9.747 | - | |
| NaCl | 8.0 | g | 7.797 | 133.424 | |
| Salt solution | 40.0 | ml | - | - | |
| Resazurin | 0.5 | mg | 4.9e-4 | 0.002 | |
| Distilled water | 960.0 | ml | - | - | |
| 1 Mix components thoroughly, bring to a boil and cool down under a stream of N2. While gassing, add the following solutions and adjust pH to 7.0: | |||||
|
L-Cysteine HCl x H2O
(5%) |
10.0 | ml | - | - | |
| 2 Distribute the medium into culture vessels (e.g., 10 ml in Balch type tubes) under N2, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions (autoclaved and stored under a N2 atmosphere): | |||||
|
Glucose
(1 M) |
6.0 | ml | - | - | |
|
Na2S2O3
(1 M) |
10.0 | ml | - | - | |
Solution occurs in 9
other media (first defined in Medium 104).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 |
| K2HPO4 | 1.00 | g | 1 | 5.741 |
| KH2PO4 | 1.00 | g | 1 | 7.348 |
| NaHCO3 | 10.00 | g | 10 | 119.038 |
| NaCl | 2.00 | g | 2 | 34.223 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |