J1243
1243 .
VULCANO LOW SALT MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1243 #
ml
Equipment needed: Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 1.70 | g | 1.735 | 29.683 | |
| MgCl2 x 2 H2O | 1.00 | g | 1.02 | 7.775 | |
| MgSO4 x 7 H2O | 1.00 | g | 1.02 | 4.14 | |
| KCl | 0.30 | g | 0.306 | 4.106 | |
| Na2SO4 | 1.00 | g | 1.02 | 7.184 | |
| (NH4)2SO4 | 0.30 | g | 0.306 | 2.317 | |
| K2HPO4 | 0.05 | g | 0.051 | 0.293 | |
| KH2PO4 | 0.05 | g | 0.051 | 0.375 | |
| CaCl2 x 2 H2O | 0.02 | g | 0.02 | 0.139 | |
| FeCl2 x 4 H2O | 0.10 | g | 0.102 | 0.513 | |
| Iron(III) citrate | 0.10 | g | 0.102 | 0.417 | |
| L-Cysteine HCl x H2O | 0.30 | g | 0.306 | - | |
| Resazurin | 1.00 | mg | 0.001 | 0.004 | |
| Trace element solution | 1.00 | ml | - | - | |
| Se-Ni-W solution | 1.00 | ml | - | - | |
| Distilled water | 966.00 | ml | - | - | |
| 1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (autoclaved): | |||||
|
Yeast extract
(10%) |
5.00 | ml | - | - | |
|
Trypticase peptone
(10%) |
5.00 | ml | - | - | |
|
Sodium acetate
(1.0 M) |
2.00 | ml | - | - | |
| 2 Adjust pH to 3.8-4.0 with HCl. | |||||
Solution occurs in 1
other media (first defined in Medium J1243).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na2SeO3 | 10 | mg | 0.1 | - |
| NiCl2 x 6 H2O | 20 | mg | 0.2 | 1.543 |
| Na2WO4 x 2 H2O | 30 | mg | 0.3 | 0.91 |
| Distilled water | 100 | ml | - | - |
Solution occurs in 38
other media (first defined in Medium J439).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(25%) |
12.5 | ml | - | - |
| FeSO4 x 7 H2O | 2.1 | g | 2.1 | 7.554 |
| H3BO3 | 30.0 | mg | 0.03 | 0.485 |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | - |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 |
| ZnSO4 x 7 H2O | 144.0 | mg | 0.144 | 0.501 |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
| Distilled water | 987.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 3.9 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |