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JCM 1246 . BICARBONATE-BUFFERED MEDIUM WITH ACETHYLENE

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1246 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.225 g 0.219 1.49
KCl 0.200 g 0.195 2.612
MgCl2 x 6 H2O 0.200 g 0.195 0.958
KH2PO4 0.020 g 0.019 0.143
FeSO4 x 6 H2O
(in 0.02 N H2SO4, 1%) 		0.100 ml - -
FeCl2 solution 1.000 ml - -
Trace element solution 1.000 ml - -
NaCl 20.000 g 19.474 333.234
NH4Cl 0.200 g 0.195 3.641
HEPES 5.200 g 5.063 21.247
Distilled water 1000.000 ml - -
1 Mix components thoroughly and adjust pH to 7.5. Distribute the medium in culture vessels (e.g., 10 ml in Balch-type tubes), flush with N2 and seal with butyl rubber stoppers. Autoclave. After cooling, add per liter the following solution (autoclaved or *filter-sterilized):
NaHCO3
(*, 8%) 		20.000 ml - -
L-Cysteine HCl x H2O
(5%) 		5.000 ml - -
2 After inoculation, add sterile pure acetylene gas, made from calcium carbide and water, to the gas phase (the first time, 0.5% volume of the gas phase: the next day, 1.0% volume: increase each day until 2.5% volume is reached).

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.474 333.234
HEPES 5.063 21.247
CaCl2 x 2 H2O 0.219 1.49
NH4Cl 0.195 3.641
KCl 0.195 2.612
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain